Anti-MAD1抗体[MAD1D6H4] (ab50791)

概述

  • 产品名称Anti-MAD1抗体[MAD1D6H4]
    参阅全部 MAD1 一抗
  • 描述
    小鼠单克隆抗体[MAD1D6H4] to MAD1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant Human MAD1

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液Preservative: 0.05% Sodium Azide
    Constituents: 1% BSA, PBS, 8mM Sodium phosphate, 3mM Potassium chloride, 140mM Sodium chloride, 1.5mM Potassium phosphate, pH 7.4
  • Concentration information loading...
  • 纯度Protein G purified
  • 纯化说明The antibody was filtered through an 0.22 µm membrane.
  • 克隆单克隆
  • 克隆编号MAD1D6H4
  • 同种型IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab50791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent dilution. Predicted molecular weight: 83 kDa.

靶标

  • 功能Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
  • 组织特异性Expressed weakly at G0/G1 and highly at late S and G2/M phase.
  • 疾病相关Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
  • 序列相似性Belongs to the MAD1 family.
  • 翻译后修饰Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
  • 细胞定位Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Information by UniProt
  • 数据库链接
  • 别名
    • hMAD1 antibody
    • HsMAD1 antibody
    • MAD1 antibody
    • MAD1 mitotic arrest deficient like 1 (yeast) antibody
    • MAD1-like protein 1 antibody
    • MAD1L1 antibody
    • MD1L1_HUMAN antibody
    • Mitotic arrest deficient 1 antibody
    • Mitotic arrest deficient 1-like protein 1 antibody
    • Mitotic checkpoint MAD1 protein homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD1 antibody
    • PIG9 antibody
    • Tax binding protein 181 antibody
    • Tax-binding protein 181 antibody
    • TP53I9 antibody
    • Tumor protein p53 inducible protein 9 antibody
    • TXBP181 antibody
    see all

Anti-MAD1 antibody [MAD1D6H4] 图像

Anti-MAD1 antibody [MAD1D6H4] (ab50791)参考文献

ab50791 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Methanol
Permeabilization No
Username

Dr. Kirk McManus

Verified customer

提交于 May 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"