概述

  • 产品名称
    Anti-MAD1抗体[9B10]
    参阅全部 MAD1 一抗
  • 描述
    小鼠单克隆抗体[9B10] to MAD1
  • 经测试应用
    适用于: IP, ICC/IF, Flow Cytmore details
    不适用于: WB
  • 种属反应性
    与反应: Human
  • 免疫原

    Recombinant full length MAD1 protein (Human)

性能

应用

Our Abpromise guarantee covers the use of ab5783 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP Use a concentration of 5 µg/ml.
ICC/IF 1/500 - 1/1000.
Flow Cyt Use 0.1µg for 106 cells.
  • 应用说明
    Is unsuitable for WB.
  • 靶标

    • 功能
      Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
    • 组织特异性
      Expressed weakly at G0/G1 and highly at late S and G2/M phase.
    • 疾病相关
      Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
    • 序列相似性
      Belongs to the MAD1 family.
    • 翻译后修饰
      Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
    • 细胞定位
      Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
    • Information by UniProt
    • 数据库链接
    • 别名
      • hMAD1 antibody
      • HsMAD1 antibody
      • MAD1 antibody
      • MAD1 mitotic arrest deficient like 1 (yeast) antibody
      • MAD1-like protein 1 antibody
      • MAD1L1 antibody
      • MD1L1_HUMAN antibody
      • Mitotic arrest deficient 1 antibody
      • Mitotic arrest deficient 1-like protein 1 antibody
      • Mitotic checkpoint MAD1 protein homolog antibody
      • Mitotic spindle assembly checkpoint protein MAD1 antibody
      • PIG9 antibody
      • Tax binding protein 181 antibody
      • Tax-binding protein 181 antibody
      • TP53I9 antibody
      • Tumor protein p53 inducible protein 9 antibody
      • TXBP181 antibody
      see all

    图片

    • ab5783 staining MAD1 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/100 in 1% PBS BSA) for 1 hour at 37°C. A goat anti-mouse IgG polyclonal was used as the secondary antibody at a dilution of 1/100.

    • Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    文献

    This product has been referenced in:
    • Rajanala K  et al. Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function. J Cell Sci 127:3505-20 (2014). Read more (PubMed: 24938596) »
    • Hewitt L  et al. Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J Cell Biol 190:25-34 (2010). ICC/IF ; Human . Read more (PubMed: 20624899) »

    See all 2 Publications for this product

    客户评价及客户问答

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Colon carcinoma)
    Gel Running Conditions
    Non-reduced Denaturing (10%)
    Loading amount
    40 µg
    Specification
    Colon carcinoma
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5µg/mL · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    提交于 Jul 06 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Colon carcinoma)
    Permeabilization
    Yes - Triton 0.5%
    Specification
    Colon carcinoma
    Fixative
    Paraformaldehyde
    Username

    Dr. Eleni Petsalaki

    Verified customer

    提交于 Jun 15 2016

    Thank you for your phone call. The originator of ab5783 has informed me that they no longer have the rabbit anti-MAD1 antibody that is mentioned in the image caption. If I can be of further assistance, please let me know.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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