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Our Abpromise guarantee covers the use of ab5783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|ICC/IF||1/500 - 1/1000.|
|Flow Cyt||Use 0.1µg for 106 cells.|
ab5783 staining MAD1 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/100 in 1% PBS BSA) for 1 hour at 37°C. A goat anti-mouse IgG polyclonal was used as the secondary antibody at a dilution of 1/100.