Anti-Macrophage specific lectin抗体[ER-MP23] (ab15635)

概述

  • 产品名称Anti-Macrophage specific lectin抗体[ER-MP23]
  • 描述
    大鼠单克隆抗体[ER-MP23] to Macrophage specific lectin
  • 特异性Detects mature macrophages in connective tissues which are situated in the vicinity of epithelia e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. The antibody blocks the mouse macrophage galactose-specific lectin (MMGL).
  • 经测试应用适用于: IHC-Fr, Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation (Mouse macrophage cell lines).

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA. pH 7.2
  • Concentration information loading...
  • 克隆单克隆
  • 克隆编号ER-MP23
  • 同种型IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab15635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 1 µg/ml.

Permeabilisation with 0.01% Triton is recommended

Flow Cyt Use 1µg for 106 cells. ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性The antigen is found on the surface of connective tissue macrophages and their precursor cells in bone marrow. Macrophage cell lines (J774-1.6, RAW309Cr.1, WR19M.1) corresponding to mature macrophages express high levels of the antigen .
  • 细胞定位Cell Membrane
  • 数据库链接
  • 别名
    • Macrophage asialoglycoprotein binding protein 1 antibody
    • Macrophage galactose specific lectin 1 antibody
    • MASGP BP antibody
    • Mgl antibody
    • Mgl1 antibody
    • MMGL antibody
    see all

Anti-Macrophage specific lectin antibody [ER-MP23] 图像

  • Overlay histogram showing RAW 264.7 cells stained with ab15635 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15635, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% paraformaldehyde used under the same conditions.
    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

Anti-Macrophage specific lectin antibody [ER-MP23] (ab15635)参考文献

This product has been referenced in:
  • Heine SJ  et al. Intradermal delivery of Shigella IpaB and IpaD type III secretion proteins: kinetics of cell recruitment and antigen uptake, mucosal and systemic immunity, and protection across serotypes. J Immunol 192:1630-40 (2014). Mouse . Read more (PubMed: 24453241) »
  • Cudejko C  et al. p16INK4a-deficiency promotes IL-4-induced polarization and inhibits pro-inflammatory signaling in macrophages. Blood : (2011). IHC-FoFr ; Mouse . Read more (PubMed: 21636855) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5%
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提交于 Jun 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"