重组
RabMAb

Anti-Macrophage Inflammatory Protein 1 beta抗体[EP521Y] (ab45690)

概述

  • 产品名称
    Anti-Macrophage Inflammatory Protein 1 beta抗体[EP521Y]
    参阅全部 Macrophage Inflammatory Protein 1 beta 一抗
  • 描述
    兔单克隆抗体[EP521Y] to Macrophage Inflammatory Protein 1 beta
  • 经测试应用
    适用于: IP, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Human
  • 免疫原

    corresponding to Human Macrophage Inflammatory Protein 1 beta aa 1 to the C-terminus (N terminal).
    Database link: P13236

  • 阳性对照
    • THP-1, Raw264.7
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

性能

应用

Our Abpromise guarantee covers the use of ab45690 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/60.
WB 1/1000 - 1/500000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
ICC/IF 1/100.

靶标

  • 功能
    Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
  • 序列相似性
    Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰
    N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
  • 细胞定位
    Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MIP 1 beta antibody
    • Secreted protein G 26 antibody
    • ACT 2 antibody
    • ACT-2 antibody
    • ACT2 antibody
    • AT744.1 antibody
    • AT744.2 antibody
    • C C motif chemokine 4 antibody
    • C C motif chemokine 4 like antibody
    • C C motif chemokine ligand 4 like 1 antibody
    • C C motif chemokine ligand 4 like 2 antibody
    • CC chemokine ligand 4 antibody
    • CC chemokine ligand 4L1 antibody
    • CC chemokine ligand 4L1d2 antibody
    • CC chemokine ligand 4L2 antibody
    • CCL4 antibody
    • CCL4_HUMAN antibody
    • ccl4l 1 antibody
    • CCL4L antibody
    • CCL4L1 antibody
    • Chemokine (C C motif) ligand 4 antibody
    • Chemokine (C C motif) ligand 4 like 1 antibody
    • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
    • Chemokine (C C motif) ligand 4 like 2 antibody
    • Chemokine CC Motif Ligand 4 antibody
    • G 26 antibody
    • G 26 T lymphocyte secreted protein antibody
    • G-26 T-lymphocyte-secreted protein antibody
    • HC21 antibody
    • Immune activation 2 antibody
    • LAG 1 antibody
    • LAG-1 antibody
    • LAG1 antibody
    • Lymphocyte activation gene 1 antibody
    • Lymphocyte activation gene 1 protein antibody
    • Macrophage inflammatory protein 1 beta antibody
    • Macrophage inflammatory protein 1-beta antibody
    • Macrophage inflammatory protein 1b2 antibody
    • MGC104418 antibody
    • MGC126025 antibody
    • MGC126026 antibody
    • MIP-1-beta antibody
    • MIP-1-beta(1-69) antibody
    • MIP-1-beta(3-69) antibody
    • MIP1 beta antibody
    • MIP1B antibody
    • MIP1B1 antibody
    • Monocyte adherence induced protein 5 alpha antibody
    • PAT 744 antibody
    • Protein H400 antibody
    • SCYA2 antibody
    • SCYA4 antibody
    • SCYA4L antibody
    • SCYA4L1 antibody
    • SCYA4L2 antibody
    • SCYQ4L2 antibody
    • Secreted protein G 26 antibody
    • Secreted protein G26 antibody
    • SIS gamma antibody
    • SIS-gamma antibody
    • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
    • Small inducible cytokine A4 antibody
    • small inducible cytokine A4-like antibody
    • Small-inducible cytokine A4 antibody
    • T cell activation protein 2 antibody
    • T-cell activation protein 2 antibody
    see all

Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] 图像

  • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untagged human CCL4 recombinant protein (aa24-92)
    Lane 2 : Untagged human CCL4L recombinant protein (aa24-92)
    Lane 3 : GST-tagged human CCL3 recombinant protein (aa27-92)
    Lane 4 : GST-tagged human CCL3L recombinant protein 2*(aa28-93)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 10 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 seconds

    Blocking/Diluting buffer and concentration 5% NFDM /TBST 

  • Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) cells labeling Macrophage Inflammatory Protein 1 beta/CCL4 + CCL4L with ab45690 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291, ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution. DAPI was used to stain nuclei blue.

    The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.

  • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untreated THP-1 (human acute monocytic leukemia) lysate
    Lane 2 : THP-1 treated with 100 nM Phorbol-12-myristate-13-acetate(PMA) overnight, then treated with Lipopolysaccharides (LPS) 100 ng/mL for 7 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours, lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 100000

    Predicted band size : 10 kDa
    Observed band size : 12 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

    Blocking/Diluting buffer and concentration 5% NFDM /TBST.

    Macrophage inflammatory protein 1 beta (CCL4) is induced in macrophages following exposure to bacterial LPS (PMID: 9848081).

  • ab45690 at 1/60 immunoprecipitating Macrophage Inflammatory Protein 1 beta/CCL4 + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).

    Lane 1 (input): THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10μg

    Lane 2 (+): ab45690 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab45690 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    For western blotting, ab45690 at 1/1000 and ab131366 VeriBlot for IP (HRP) was used as the secondary antibody (1/1000).

    Blocking/Diluting buffer 5% NFDM/TBST

  • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690) at 1/1000 dilution

    Lane 1 : Untreated Raw264.7 (mouse abelson murine leukemia virus-induced tumor) whole cell lysate
    Lane 2 : Raw264.7 (mouse abelson murine leukemia virus-induced tumor) treated with LPS 10µg/mL for 4 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size : 10 kDa


    Exposure time : 3 minutes

    Blocking/Diluting buffer and concentration 5% NFDM /TBST 

Anti-Macrophage Inflammatory Protein 1 beta antibody [EP521Y] (ab45690)参考文献

ab45690 has not yet been referenced specifically in any publications.

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