RabMAb

Anti-Macrophage Inflammatory Protein 1 beta抗体[EP494Y] (ab40857)

概述

  • 产品名称
    Anti-Macrophage Inflammatory Protein 1 beta抗体[EP494Y]
    参阅全部 Macrophage Inflammatory Protein 1 beta 一抗
  • 描述
    兔单克隆抗体[EPR494] to Macrophage Inflammatory Protein 1 beta
  • 经测试应用
    适用于: IP, ICC/IF, WBmore details
    不适用于: IHC
  • 种属反应性
    与反应: Human
  • 免疫原

    corresponding to Human Macrophage Inflammatory Protein 1 beta aa 50 to the C-terminus (C terminal).
    Database link: P13236

  • 阳性对照
    • THP-1 cell lysate
  • 常规说明

    This product is a recombinant rabbit monoclonal antibody.

     

     

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

性能

应用

Our Abpromise guarantee covers the use of ab40857 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IP 1/50.
ICC/IF 1/100.
WB 1/1000 - 1/500000. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
  • 应用说明
    Is unsuitable for IHC.
  • 靶标

    • 功能
      Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
    • 序列相似性
      Belongs to the intercrine beta (chemokine CC) family.
    • 翻译后修饰
      N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
    • 细胞定位
      Secreted.
    • Information by UniProt
    • 数据库链接
    • 别名
      • MIP 1 beta antibody
      • Secreted protein G 26 antibody
      • ACT 2 antibody
      • ACT-2 antibody
      • ACT2 antibody
      • AT744.1 antibody
      • AT744.2 antibody
      • C C motif chemokine 4 antibody
      • C C motif chemokine 4 like antibody
      • C C motif chemokine ligand 4 like 1 antibody
      • C C motif chemokine ligand 4 like 2 antibody
      • CC chemokine ligand 4 antibody
      • CC chemokine ligand 4L1 antibody
      • CC chemokine ligand 4L1d2 antibody
      • CC chemokine ligand 4L2 antibody
      • CCL4 antibody
      • CCL4_HUMAN antibody
      • ccl4l 1 antibody
      • CCL4L antibody
      • CCL4L1 antibody
      • Chemokine (C C motif) ligand 4 antibody
      • Chemokine (C C motif) ligand 4 like 1 antibody
      • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
      • Chemokine (C C motif) ligand 4 like 2 antibody
      • Chemokine CC Motif Ligand 4 antibody
      • G 26 antibody
      • G 26 T lymphocyte secreted protein antibody
      • G-26 T-lymphocyte-secreted protein antibody
      • HC21 antibody
      • Immune activation 2 antibody
      • LAG 1 antibody
      • LAG-1 antibody
      • LAG1 antibody
      • Lymphocyte activation gene 1 antibody
      • Lymphocyte activation gene 1 protein antibody
      • Macrophage inflammatory protein 1 beta antibody
      • Macrophage inflammatory protein 1-beta antibody
      • Macrophage inflammatory protein 1b2 antibody
      • MGC104418 antibody
      • MGC126025 antibody
      • MGC126026 antibody
      • MIP-1-beta antibody
      • MIP-1-beta(1-69) antibody
      • MIP-1-beta(3-69) antibody
      • MIP1 beta antibody
      • MIP1B antibody
      • MIP1B1 antibody
      • Monocyte adherence induced protein 5 alpha antibody
      • PAT 744 antibody
      • Protein H400 antibody
      • SCYA2 antibody
      • SCYA4 antibody
      • SCYA4L antibody
      • SCYA4L1 antibody
      • SCYA4L2 antibody
      • SCYQ4L2 antibody
      • Secreted protein G 26 antibody
      • Secreted protein G26 antibody
      • SIS gamma antibody
      • SIS-gamma antibody
      • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
      • Small inducible cytokine A4 antibody
      • small inducible cytokine A4-like antibody
      • Small-inducible cytokine A4 antibody
      • T cell activation protein 2 antibody
      • T-cell activation protein 2 antibody
      see all

    Anti-Macrophage Inflammatory Protein 1 beta antibody [EP494Y] 图像

    • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP494Y] (ab40857) at 1/10000 dilution

      Lane 1 : Untagged human CCL4 recombinant protein (aa24-92)
      Lane 2 : Untagged human CCL4L recombinant protein (aa24-92)
      Lane 3 : GST-tagged human CCL3 recombinant protein (aa27-92)
      Lane 4 : GST-tagged human CCL3L recombinant protein 2*(aa28-93)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 10 kDa
      Observed band size : 12 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 seconds

      Blocking/Diluting buffer 5% NFDM /TBST 

    • Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling Macrophage Inflammatory Protein 1 beta/CCL4 + CCL4L with ab40857 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200. Nuclei were counterstained with DAPI (blue).

      The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.

    • All lanes : Anti-Macrophage Inflammatory Protein 1 beta antibody [EP494Y] (ab40857) at 1/1000 dilution

      Lane 1 : Untreated THP-1 (human acute monocytic leukemia) lysate
      Lane 2 : THP-1 (human acute monocytic leukemia) treated with 100 nM Phorbol-12-myristate-13-acetate(PMA) overnight, then treated with Lipopolysaccharides (LPS) 100 ng/mL for 7 hours and then 1 µg/mL Brefeldin A was added for the last 3 hours, lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size : 10 kDa
      Observed band size : 12 kDa (why is the actual band size different from the predicted?)


      Exposure time : 15 seconds

      Blocking/Diluting buffer 5% NFDM /TBST.

      Macrophage inflammatory protein 1 beta (CCL4) is induced in macrophages following exposure to bacterial LPS (PMID: 9848081).

    • ab40857 at 1/50 immunoprecipitating Macrophage Inflammatory Protein 1 beta/CCL4 + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).

      Lane 1 (input): THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10μg

      Lane 2 (+): ab40857 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab40857 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

      For western blotting: ab40857 at 1/1000 followed by ab131366 VeriBlot for IP (HRP) at 1/000 secondary antibody.

      Blocking buffer and concentration: 5% NFDM/TBST.
      Diluting buffer and concentration: 5% NFDM /TBST.

    Anti-Macrophage Inflammatory Protein 1 beta antibody [EP494Y] (ab40857)参考文献

    ab40857 has not yet been referenced specifically in any publications.

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