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Our Abpromise guarantee covers the use of ab9675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
The detection limit for recombinant Human MIP-1β is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml.
Use this antibody as the capture antibody and ab84260 as the detection antibody in sandwich ELISA (using 100 µl/well antibody solution) to detect at least 0.2 - 0.4 ng/well of recombinant Human MIP-1β.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"