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Highly pure (>98%) recombinant hMIP-1-beta (Human macrophage inflammatory protein-1 beta)
Our Abpromise guarantee covers the use of ab9675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.2 µg/ml.
The detection limit for recombinant Human MIP-1β is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions.
|Neutralising||Use a concentration of 1.75 - 3 µg/ml.
To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/ml), a concentration of 1.75 - 3 µg/ml of antibody is required.
|IHC-P||Use a concentration of 1 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Human pancreas adenocarcinoma can be used as a positive control. Incubate at 1 mg/ml for 2 hr at RT. An HRP-labeled polymer detection system was used with a DAB chromogen.
|Sandwich ELISA||Use a concentration of 0.5 - 2 µg/ml.
Use this antibody as the capture antibody and ab84260 as the detection antibody in sandwich ELISA (using 100 µl/well antibody solution) to detect at least 0.2 - 0.4 ng/well of recombinant Human MIP-1β.