Anti-Macrophage Inflammatory Protein 1 beta抗体(ab9675)

概述

  • 产品名称Anti-Macrophage Inflammatory Protein 1 beta抗体
    参阅全部 Macrophage Inflammatory Protein 1 beta 一抗
  • 描述
    兔多克隆抗体to Macrophage Inflammatory Protein 1 beta
  • 经测试应用适用于: WB, Neutralising, IHC-P, Sandwich ELISAmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Highly pure (>98%) recombinant hMIP-1-beta (Human macrophage inflammatory protein-1 beta)

性能

应用

Our Abpromise guarantee covers the use of ab9675 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 0.1 - 0.2 µg/ml.

The detection limit for recombinant Human MIP-1β is 1.5 - 3.0 ng/lane under either reducing or non-reducing conditions.

Neutralising Use a concentration of 1.75 - 3 µg/ml.

To yield one-half maximal inhibition [ND50] of the biological activity of hMIP-1-beta (50.00 ng/ml), a concentration of 1.75 - 3 µg/ml of antibody is required.

IHC-P Use a concentration of 1 mg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Human pancreas adenocarcinoma can be used as a positive control.  Incubate at 1 mg/ml for 2 hr at RT.  An HRP-labeled polymer detection system was used with a DAB chromogen.

Sandwich ELISA Use a concentration of 0.5 - 2 µg/ml.

Use this antibody as the capture antibody and ab84260 as the detection antibody in sandwich ELISA (using 100 µl/well antibody solution) to detect at least 0.2 - 0.4 ng/well of recombinant Human MIP-1β.

靶标

  • 功能Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
  • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
  • 翻译后修饰N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • MIP 1 beta antibody
    • Secreted protein G 26 antibody
    • ACT 2 antibody
    • ACT-2 antibody
    • ACT2 antibody
    • AT744.1 antibody
    • AT744.2 antibody
    • C C motif chemokine 4 antibody
    • C C motif chemokine 4 like antibody
    • C C motif chemokine ligand 4 like 1 antibody
    • CC chemokine ligand 4 antibody
    • CC chemokine ligand 4L1 antibody
    • CC chemokine ligand 4L1d2 antibody
    • CC chemokine ligand 4L2 antibody
    • CCL4 antibody
    • CCL4_HUMAN antibody
    • ccl4l 1 antibody
    • CCL4L antibody
    • CCL4L1 antibody
    • Chemokine (C C motif) ligand 4 antibody
    • Chemokine (C C motif) ligand 4 like 1 antibody
    • Chemokine (C C motif) ligand 4 like 1, telomeric antibody
    • Chemokine (C C motif) ligand 4 like 2 antibody
    • Chemokine CC Motif Ligand 4 antibody
    • G 26 antibody
    • G 26 T lymphocyte secreted protein antibody
    • G-26 T-lymphocyte-secreted protein antibody
    • HC21 antibody
    • Immune activation 2 antibody
    • LAG 1 antibody
    • LAG-1 antibody
    • LAG1 antibody
    • Lymphocyte activation gene 1 antibody
    • Lymphocyte activation gene 1 protein antibody
    • Macrophage inflammatory protein 1 beta antibody
    • Macrophage inflammatory protein 1-beta antibody
    • Macrophage inflammatory protein 1b2 antibody
    • MGC104418 antibody
    • MGC126025 antibody
    • MGC126026 antibody
    • MIP-1-beta antibody
    • MIP-1-beta(1-69) antibody
    • MIP-1-beta(3-69) antibody
    • MIP1 beta antibody
    • MIP1B antibody
    • MIP1B1 antibody
    • Monocyte adherence induced protein 5 alpha antibody
    • PAT 744 antibody
    • Protein H400 antibody
    • SCYA2 antibody
    • SCYA4 antibody
    • SCYA4L antibody
    • SCYA4L1 antibody
    • SCYQ4L2 antibody
    • Secreted protein G 26 antibody
    • Secreted protein G26 antibody
    • SIS gamma antibody
    • SIS-gamma antibody
    • Small inducible cytokine A4 (homologous to mouse Mip 1b) antibody
    • Small inducible cytokine A4 antibody
    • small inducible cytokine A4-like antibody
    • Small-inducible cytokine A4 antibody
    • T cell activation protein 2 antibody
    • T-cell activation protein 2 antibody
    see all

Anti-Macrophage Inflammatory Protein 1 beta antibody (ab9675)参考文献

This product has been referenced in:
  • Sakala IG  et al. Dipterinyl calcium pentahydrate inhibits intracellular mycobacterial growth in human monocytes via the C-C chemokine MIP-1ß and nitric oxide. Infect Immun 81:1974-83 (2013). Neutralising ; Human . Read more (PubMed: 23509148) »

See 1 Publication for this product

Product Wall



ab9675 and ab9847 are not conjugated. If you would like to conjugate them to fluorochromes, we have easy kits which would allow you to do this. Please see: http://www.abcam.com/easylink

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"