Mouse MIP1 beta neutralizes the bioactivity of recombinant, mouse MIP1 beta, but not that of recombinant, human MIP1 beta, recombinant, human MIP1 alpha or recombinant, mouse MIP1 alpha.
In indirect ELISA and immunoblotting, this antibody displays less than 2% cross-reactivity with recombinant, mouse MIP1 alpha, recombinant, human MIP1 alpha and recombinant, human MIP1 alpha.
The product shows no cross-reactivity with other tested cytokines using indirect ELISA.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at a concentration of 0.5 µg/ml to detect <1ng/well of recombinant mouse MIP1 beta.
Neut: Use at a concentration of 50 - 100 µg/ml.
WB: Use at a concentration of 1 µg/ml to detect recombinant mouse MIP1 beta at 5ng/lane under reducing conditions.
Predicted molecular weight: 11 kDa.
Anti-Mouse MIP1 beta was tested for its ability to neutralize the bioactivity of recombinant, mouse MIP1 beta in an in vitro colony forming assay using hematopoietic stem cells.
The ND50 of the antibody is defined as the concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, mouse MIP1 beta, which is present at five times its own EC50 (the concentration of recombinant, mouse MIP1 beta producing a one-half maximal bioactivity without antibody).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Belongs to the intercrine beta (chemokine CC) family.
N-terminal processed form MIP-1-beta(3-69) is produced by proteolytic cleavage after secretion from peripheral blood lymphocytes.
Johannsen A et al. Definition of Key Variables for the Induction of Optimal NY-ESO-1-Specific T Cells in HLA Transgene Mice. J Immunol185:3445-55 (2010).
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