Anti-Macrophage Inflammatory Protein 1 alpha / CCL3抗体(ab9781)

Abreviews (1)Q&A (1)Specific References (4)
Customer reviews and FAQs

概述

  • 产品名称Anti-Macrophage Inflammatory Protein 1 alpha / CCL3抗体
    参阅全部 Macrophage Inflammatory Protein 1 alpha / CCL3 一抗
  • 描述
    兔多克隆抗体to Macrophage Inflammatory Protein 1 alpha / CCL3
  • 经测试应用适用于: Indirect ELISA, WB, ELISA, Neutralising, IHC-Frmore details
  • 种属反应性
    与反应: Rat
  • 免疫原

    Highly pure (>98%) recombinant rMIP-1-alpha (Rat macrophage inflammatory protein-1 alpha)

性能

应用

Our Abpromise guarantee covers the use of ab9781 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Indirect ELISA
WB
ELISA
Neutralising
IHC-Fr
  • 应用说明Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of rMIP-1a (100 ng/ml), a concentration of 8.0 - 10.0 µg/ml of this antibody is required.

    ELISA:To detect rMIP-1-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rMIP-1-alpha.

    Western Blot:To detect rMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

    • 靶标

    • 功能Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
    • 序列相似性Belongs to the intercrine beta (chemokine CC) family.
    • 翻译后修饰N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
    • 细胞定位Secreted.
    • Information by UniProt
    • 数据库链接
      • 别名
        • C C motif chemokine 3 antibody
        • CCL 3 antibody
        • CCL3 antibody
        • CCL3_HUMAN antibody
        • Chemokine C C motif ligand 3 antibody
        • G0/G1 switch regulatory protein 19 1 antibody
        • G0/G1 switch regulatory protein 19-1 antibody
        • G0S19 1 antibody
        • G0S19 1 protein antibody
        • Heparin binding chemotaxis protein antibody
        • L2G25B antibody
        • LD78 alpha antibody
        • LD78-alpha(4-69) antibody
        • LD78alpha antibody
        • Macrophage inflammatory protein 1 alpha antibody
        • Macrophage inflammatory protein 1-alpha antibody
        • MIP 1 alpha antibody
        • MIP 1A antibody
        • MIP-1-alpha antibody
        • MIP-1-alpha(4-69) antibody
        • MIP1A antibody
        • PAT 464.1 antibody
        • SCYA 3 antibody
        • SCYA3 antibody
        • SIS alpha antibody
        • SIS beta antibody
        • SIS-beta antibody
        • Small inducible cytokine A3 antibody
        • small inducible cytokine A3 (homologous to mouse Mip-1a) antibody
        • Small-inducible cytokine A3 antibody
        • Tonsillar lymphocyte LD78 alpha protein antibody
        see all

      Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody 图像

      • Indirect ELISA - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9781)
        Indirect ELISA - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9781)Image from Tripathy D & Grammas P,J Neuroinflammation. 2009 Mar 16;6:10. Fig 5.; doi:10.1186/1742-2094-6-10; 16 March 2009, Journal of Neuroinflammation 2009, 6:10
        Indirect ELISA measuring Macrophage Inflammatory Protein 1 alpha / CCL3 levels using ab9781.

        Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.

      Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9781)参考文献

      This product has been referenced in:
      • Johnson EA  et al. Increased expression of the chemokines CXCL1 and MIP-1a by resident brain cells precedes neutrophil infiltration in the brain following prolonged soman-induced status epilepticus in rats. J Neuroinflammation 8:41 (2011). IHC-Fr ; Rat . Read more (PubMed: 21535896) »
      • Tripathy D & Grammas P Acetaminophen protects brain endothelial cells against oxidative stress. Microvasc Res 77:289-96 (2009). ELISA ; Rat . Read more (PubMed: 19265712) »

      See all 4 Publications for this product

      发表研究结果有使用 ab9781？请让我们知道，以便我们可以引用本数据表中的参考文章。

      Product Wall

      提交 ab9781 的 abreview 提交 ab9781 的问题

      2 Abreviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody

       Good
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application Western blot
      Sample Rat Tissue lysate - whole (liver)
      Loading amount 30 µg
      Specification liver
      Treatment LPS 1 hour
      Gel Running Conditions Reduced Denaturing
      Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      abreview image
      Username

      Dr. Haoshu Fang

      Verified customer

      提交于 Nov 19 2012

      I would need to know whether any of the anti-mouse CCL3 sera that you list below were shown to be neutralizing ? And if you’d sell batches of higher quantities as this would be for in vivo experiments and related prices ?




      ...

      Read More

      Thank you for contacting Abcam.

      All of the antibodies that you mentioned are guaranteed under our Abpromise to work in a Neutralizing assay, in various species:

      ab89546 - Works human samples
      ab9673 – Works in human samples Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"