Anti-Macrophage Inflammatory蛋白1 alpha / CCL3抗体(ab9781)
- 数据表
- 具体的参考文献 (4)
- 实验方案
概述
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产品名称Anti-Macrophage Inflammatory蛋白1 alpha / CCL3抗体
参阅全部 Macrophage Inflammatory Protein 1 alpha / CCL3 一抗 -
描述兔多克隆抗体to Macrophage Inflammatory蛋白1 alpha / CCL3
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宿主Rabbit
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经测试应用适用于: Indirect ELISA, WB, ELISA, Neutralising, IHC-Frmore details
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种属反应性与反应: Rat
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免疫原
Highly pure (>98%) recombinant rMIP-1-alpha (Rat macrophage inflammatory protein-1 alpha)
性能
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形式Lyophilised:Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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存储溶液PBS, pH 7.4, no preservative, sterile filtered
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Concentration information loading... -
纯度Immunogen affinity purified
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克隆多克隆
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同种型unknown
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轻链类型unknown
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研究领域
相关产品
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Compatible Secondaries
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab9781 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| Indirect ELISA | ||
| WB | ||
| ELISA | ||
| Neutralising | ||
| IHC-Fr |
应用说明
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of rMIP-1a (100 ng/ml), a concentration of 8.0 - 10.0 µg/ml of this antibody is required.
ELISA:To detect rMIP-1-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rMIP-1-alpha.
Western Blot:To detect rMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
ELISA:To detect rMIP-1-alpha by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant rMIP-1-alpha.
Western Blot:To detect rMIP-1-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rMIP-1-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
靶标
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功能Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
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序列相似性Belongs to the intercrine beta (chemokine CC) family.
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翻译后修饰N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 25542 Rat
- SwissProt: P50229 Rat
- Unigene: 10139 Rat
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别名
- C C motif chemokine 3 antibody
- CCL 3 antibody
- CCL3 antibody
see all
图片
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Indirect ELISA - Anti-Macrophage Inflammatory Protein 1 alpha / CCL3 antibody (ab9781)Image from Tripathy D & Grammas P,J Neuroinflammation. 2009 Mar 16;6:10. Fig 5.; doi:10.1186/1742-2094-6-10; 16 March 2009, Journal of Neuroinflammation 2009, 6:10Indirect ELISA measuring Macrophage Inflammatory Protein 1 alpha / CCL3 levels using ab9781.
Supernatants collected from rat cerebral cortical cells after various treatments were coated onto 96-well flat bottom plates and incubated overnight at 4°C. The plates were blocked with 1% BSA at 37°C for 45 min before incubation with primary antibody (1/1000) in bicarbonate buffer overnight at 4°C. An HRP-conjugated secondary IgG (1/1000) was added and incubated for 45 min at 37°C, in the dark. The reaction was developed by adding o-phenylene diamine H2O2 for 20 min. Optical density was measured at 450 nm.
文献
This product has been referenced in:
- Johnson EA et al. Increased expression of the chemokines CXCL1 and MIP-1a by resident brain cells precedes neutrophil infiltration in the brain following prolonged soman-induced status epilepticus in rats. J Neuroinflammation 8:41 (2011). IHC-Fr ; Rat . Read more (PubMed: 21535896) »
- Tripathy D & Grammas P Acetaminophen protects brain endothelial cells against oxidative stress. Microvasc Res 77:289-96 (2009). ELISA ; Rat . Read more (PubMed: 19265712) »
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"