In ELISA formats and other immunoreactive assays, this antibody will recognize recombinant and native human MIP-1a present in body fluids and cell supernatants. This antiserum has not been evaluated for its ability to stain human MIP-1a in tissue sections, nor for its ability to neutralize human MIP-1a in bioassays, nor for its performance in immunoblot analysis.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: 1/1000 - 1/2000.
ELISA will detect human MIP-1a from supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human serum plus 10 ng/mL E.coli LPS.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Belongs to the intercrine beta (chemokine CC) family.
N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.