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Tissue/ cell preparation (Human). Human monocytes
Our Abpromise guarantee covers the use of ab22506 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
Membrane permeabilisation is required for this application.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-Fr||1/100 - 1/200.|
|IHC-P||Use a concentration of 1 µg/ml.|
ab22506 staining Macrophages in human spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBF and blocked with 0.250% casein for 5 minutes at 25°C; antigen retrieval was by proteinase K. Samples were incubated with primary antibody (1/5000 in Tris-HCL) for 30 minutes at 25°C. A HRP-conjugated labeled polymer was used as the secondary antibody.
ab22506 staining Macrophages in human brain tissue section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone and blocked for 30 minutes at 25°C. The primary antibody was diluted, 1/100 and incubated with sample for 1 hours at 37°C. A Biotin conjugated goat polyclonal to mouse IgG1, diluted 1/250 was used as secondary. The Biotinylated sites were visualized with Invitrogen streptavidin-Alexa546.
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