概述

  • 产品名称
    Anti-MACROD1抗体
    参阅全部 MACROD1 一抗
  • 描述
    兔多克隆抗体to MACROD1
  • 宿主
    Rabbit
  • 经测试应用
    适用于: ICC/IF, IHC-Pmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    antigen sequence: SFLKGLSDKQ REEHYFCKDF VRLKKIPTWK EMAKGVAVKV EEPRYKKDKQ LNEKISLLRS DITKLEV, corresponding to amino acids of Human MACROD1.

  • 阳性对照
    • Human colon tissue.

应用

Our Abpromise guarantee covers the use of ab122688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能
    Plays a role in estrogen signaling. Binds to androgen receptor (AR) and amplifies the transactivation function of AR in response to androgen. May play an important role in carcinogenesis and/or progression of hormone-dependent cancers by feed-forward mechanism that activates ESR1 transactivation. Could be an ESR1 coactivator, providing a positive feedback regulatory loop for ESR1 signal transduction. Could be involved in invasive growth by down-regulating CDH1 in endometrial cancer cells. Enhances ESR1-mediated transcription activity.
  • 疾病相关
    Note=A chromosomal aberration involving MACROD1 is found in acute leukemia. Translocation t(11;21)(q13;q22) that forms a RUNX1-MACROD1 fusion protein.
  • 序列相似性
    Contains 1 Macro domain.
  • Information by UniProt
  • 数据库链接
  • 别名
    • LRP16 antibody
    • MACD1_HUMAN antibody
    • MACRO domain containing 1 antibody
    • MACRO domain-containing protein 1 antibody
    • Macrod1 antibody
    • O-acetyl-ADP-ribose deacetylase MACROD1 antibody
    • Protein LRP16 antibody
    see all

图片

  • Immunofluorescent staining of Human cell line A-431 shows positivity in mitochondria. Recommended concentration of ab122688 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab122688, at 1/200 dilution, staining MACROD1 in paraffin-embedded Human colon tissue by Immunohistochemistry.

文献

This product has been referenced in:
  • Agnew T  et al. MacroD1 Is a Promiscuous ADP-Ribosyl Hydrolase Localized to Mitochondria. Front Microbiol 9:20 (2018). Read more (PubMed: 29410655) »
  • Ahmed S  et al. Loss of the Mono-ADP-ribosyltransferase, Tiparp, Increases Sensitivity to Dioxin-induced Steatohepatitis and Lethality. J Biol Chem 290:16824-40 (2015). Read more (PubMed: 25975270) »

See all 2 Publications for this product

客户评价及客户问答

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Prostate)
Gel Running Conditions
Reduced Denaturing (10% Gel)
Loading amount
100 µg
Specification
Prostate
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

William

Verified customer

提交于 Mar 15 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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