概述

  • 产品名称Anti-Macro H2A.2抗体
    参阅全部 Macro H2A.2 一抗
  • 描述
    兔多克隆抗体to Macro H2A.2
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Macro H2A.2.

    (Peptide available as ab15023.)

  • 阳性对照
    • This antibody gave a positive signal in the following cell lysates: HeLa nuclear extract.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 纯度Immunogen affinity purified
  • 克隆多克隆
  • 同种型IgG1
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
  • 应用说明WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 40 kDa). Can be blocked with Macro H2A.2 peptide (ab15023).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
    • 序列相似性Contains 1 histone H2A domain.
      Contains 1 Macro domain.
    • 细胞定位Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
    • Information by UniProt
    • 数据库链接
    • 别名
      • Core histone macro H2A2.2 antibody
      • Core histone macro-H2A.2 antibody
      • Core histone macroH2A2.2 antibody
      • H2A histone family member Y2 antibody
      • H2AFY2 antibody
      • H2AW_HUMAN antibody
      • Histone macroH2A2 antibody
      • Macro H2A2 antibody
      • MacroH2A.2 antibody
      • MacroH2A2 antibody
      • mH2A2 antibody
      see all

    Anti-Macro H2A.2 antibody 图像

    • Lane 1 : 2 ug of chicken erythrocyte chromatin fraction stained with ponceau.
      Lane 2 : 2 ug of chicken liver chromatin fraction stained with ponceau.
      Lanes 3 - 4 : Anti-Macro H2A.2 antibody (ab4173) at 1/500 dilution

      Lane 1 : As above
      Lane 2 : As above
      Lane 3 : 2 ug of chicken erythrocyte chromatin fraction.
      Lane 4 : 2 ug of chicken liver chromatin fraction.

      Secondary
      Lanes 3 - 4 : Secondary antibody used at a dilution of 1/3000.


      Performed under reducing conditions.

      Predicted band size : 40 kDa

      This image is courtesy of Juan Ausio

      CL = chicken liver and CE= chicken erythrocyte. Approximately 2 micrograms were loaded in each lane, blotted and reacted with primary antibody at dilution of 1:500 and 1:3000 for the secondary.

      Previously, the problem has been that macroH2A2 is not very ubiquitous compared to macro H2A.1. While this makes it good for commercialization purposes it is hard to prepare chromatin fractions that are enriched in this fraction. However, these images show a ponceau stain (left hand side blot) and the western using the ab4173 antibody. There is one specific band.

    • All lanes : Anti-Macro H2A.2 antibody (ab4173) at 1 µg/ml

      Lane 1 : HeLa nuclear extract
      Lane 2 : HeLa nuclear extract with Human Macro H2A.2 peptide (ab15023) at 1 µg/ml

      Lysates/proteins at 20 µg per lane.


      Predicted band size : 40 kDa
      Observed band size : 50 kDa (why is the actual band size different from the predicted?)
      ab4173 specifically recognises Macro H2A.2 at approximately 50 kDa (lane1), which can be blocked using the immunizing peptide (lane2). Macro H2A.2 is known to migrate slower than its predicted molecular weight of 40 kDa (PMID: 1529340).

    Anti-Macro H2A.2 antibody (ab4173)参考文献

    This product has been referenced in:
    • Yildirim O  et al. A System for Genome-Wide Histone Variant Dynamics In ES Cells Reveals Dynamic MacroH2A2 Replacement at Promoters. PLoS Genet 10:e1004515 (2014). CHIPseq ; Mouse . Read more (PubMed: 25102063) »
    • Boulard M  et al. Histone variant macroH2A1 deletion in mice causes female-specific steatosis. Epigenetics Chromatin 3:8 (2010). WB ; Mouse . Read more (PubMed: 20359320) »

    See all 2 Publications for this product

    Product Wall

    I am very pleased to hear you would like to accept our offer and test ab4173 & ab102126 in mouse samples. This code will be worth the price you pay for ab4173 & ab102126 and can be used with any single future primary antibody purchase before the expira...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"