Anti-Macro H2A.2抗体(ab102126)

概述

  • 产品名称
    Anti-Macro H2A.2抗体
    参阅全部 Macro H2A.2 一抗
  • 描述
    兔多克隆抗体to Macro H2A.2
  • 经测试应用
    适用于: WB, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Human
    预测可用于: Rat, Cow
  • 免疫原

    Recombinant protein fragment contain a sequence corresponding to a region within amino acids 197-340 of Human Macro H2A.2 (AAH16172).

  • 阳性对照
    • A431 whole cell lysate, TOV-21G xenograft

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 20% Glycerol, 1% BSA, PBS, pH 7
  • Concentration information loading...
  • 纯度
    Immunogen affinity purified
  • 克隆
    多克隆
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab102126 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/3000. Predicted molecular weight: 40 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval using heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0)

靶标

  • 功能
    Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in stable X chromosome inactivation.
  • 序列相似性
    Contains 1 histone H2A domain.
    Contains 1 Macro domain.
  • 细胞定位
    Nucleus. Chromosome. Enriched in inactive X chromosome chromatin and in senescence-associated heterochromatin.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Core histone macro H2A2.2 antibody
    • Core histone macro-H2A.2 antibody
    • Core histone macroH2A2.2 antibody
    • H2A histone family member Y2 antibody
    • H2AFY2 antibody
    • H2AW_HUMAN antibody
    • Histone macroH2A2 antibody
    • Macro H2A2 antibody
    • MacroH2A.2 antibody
    • MacroH2A2 antibody
    • mH2A2 antibody
    see all

图片

  • Anti-Macro H2A.2 antibody (ab102126) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size : 40 kDa
  • Immunohistochemical analysis of paraffin-embedded TOV-21G xenograft, using ab102126 at 1/100 dilution.

文献

ab102126 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
ChIP
Sample
Mouse Tissue lysate - nuclear (Brain nuclei)
Negative control
IgG
Specification
Brain nuclei
Detection step
Other
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 5 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.5% Fromaldehyde
Positive control
H3
Username

Abcam user community

Verified customer

提交于 Feb 10 2017

Thank you very much for your reply.

All of the information we have for each of our products is listed on the online datasheet and these are updated as soon as we receive any new information. So once we know the identity of the band, you will...

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Thank you very much for your enquiry.

Please accept my apologies for the long delay of my reply. I have been in contact with the source of this antibody regarding the additional band between 72 and 55 kDa. We suspect that this band may be du...

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Application
ChIP
Sample
Mouse Cell lysate - whole cell (ES)
Specification
ES
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
No
Negative control
macroH2a2-/- ES cells
IgG ab
Username

Abcam user community

Verified customer

提交于 Nov 09 2011

I am very pleased to hear you would like to accept our offer and test ab4173 & ab102126 in mouse samples. This code will be worth the price you pay for ab4173 & ab102126 and can be used with any single future primary antibody purchase before the expira...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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