Anti-MACF1抗体(ab117418)
Key features and details
- Rabbit polyclonal to MACF1
- Suitable for: IHC-P, ICC/IF, WB, IP, ELISA
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-MACF1抗体
参阅全部 MACF1 一抗 -
描述
兔多克隆抗体to MACF1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC/IF, WB, IP, ELISAmore details -
种属反应性
与反应: Mouse
预测可用于: Rat -
免疫原
Synthetic peptide corresponding to Mouse MACF1 aa 3650-3850.
Database link: Q9QXZ0 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Constituent: Proprietary component -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117418于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
IHC-P |
1/250.
|
|
ICC/IF |
1/250.
|
|
WB |
1/500. Predicted molecular weight: 838 kDa.
|
|
IP |
1/200.
|
|
ELISA |
1/10000.
|
说明 |
---|
IHC-P
1/250. |
ICC/IF
1/250. |
WB
1/500. Predicted molecular weight: 838 kDa. |
IP
1/200. |
ELISA
1/10000. |
靶标
-
相关性
Microtubule-actin crosslinking factor 1 (MACF1/ACF7) belongs to spectraplakin family of proteins which also includes mammalian Bpag1/dystonin, Drosophila Short Stop (Shot) and Caenorhabditis elegans Vab-10. These are large actin-microtubule linker molecules that are essential for various biological processes such as neuronal degeneration, wound healing, gastrulation. MACF1, located on mouse chromosome 4D2.2, was found to play an important role during neurogenesis- it was shown that MACF1 regulates the organization of neuronal microtubules which is essential for axon extension. Furthermore, MACF1 is required in controlling focal adhesion assembly and dynamics. MACF1 behaves as an actin-regulated ATPase and it has the ability to target microtubules that track along F-actin to focal adhesions. MACF1 was also implicated in regulating filopodia formation. -
细胞定位
Cytoplasmic, microtubule. Plasma membrane. Golgi apparatus. -
数据库链接
- Entrez Gene: 11426 Mouse
- Entrez Gene: 362587 Rat
- SwissProt: Q9QXZ0 Mouse
- SwissProt: D3ZHV2 Rat
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别名
- ABP620 antibody
- Acf7 antibody
- Aclp7 antibody
see all
图片
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
文献 (7)
ab117418 被引用在 7 文献中.
- Li D et al. Targeting long noncoding RNA PMIF facilitates osteoprogenitor cells migrating to bone formation surface to promote bone formation during aging. Theranostics 11:5585-5604 (2021). PubMed: 33859765
- Yin C et al. MACF1 alleviates aging-related osteoporosis via HES1. J Cell Mol Med N/A:N/A (2021). PubMed: 34133068
- Zhao F et al. Mesenchymal MACF1 Facilitates SMAD7 Nuclear Translocation to Drive Bone Formation. Cells 9:N/A (2020). PubMed: 32143362
- Lin X et al. Microtubule actin crosslinking factor 1 (MACF1) knockdown inhibits RANKL-induced osteoclastogenesis via Akt/GSK3ß/NFATc1 signalling pathway. Mol Cell Endocrinol 494:110494 (2019). PubMed: 31260729
- Oury J et al. MACF1 links Rapsyn to microtubule- and actin-binding proteins to maintain neuromuscular synapses. J Cell Biol 218:1686-1705 (2019). PubMed: 30842214
- Yin C et al. Mechanical unloading reduces microtubule actin crosslinking factor 1 expression to inhibit β-catenin signaling and osteoblast proliferation. J Cell Physiol 233:5405-5419 (2018). PubMed: 29219183
- Xie SD et al. [MACF1 knockdown in glioblastoma multiforme cells increases temozolomide-induced cytotoxicity]. Nan Fang Yi Ke Da Xue Xue Bao 37:1183-1189 (2017). PubMed: 28951359