概述

  • 产品名称Anti-MACC1抗体
    参阅全部 MACC1 一抗
  • 描述
    兔多克隆抗体to MACC1
  • 经测试应用适用于: WB, IHC-P, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human MACC1 (internal sequence). MACC1 antibody was raised against a 17 amino acid synthetic peptide near the center of human MACC1
    Database link: Q6ZN28

  • 阳性对照
    • Mouse liver tissue lysate; Human liver tissue IF: A431 cell line

性能

应用

Our Abpromise guarantee covers the use of ab106579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 97 kDa.
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.

靶标

  • 功能Acts as a transcription activator for MET and as a key regulator of HGF-MET signaling. Promotes cell motility, proliferation and hepatocyte growth factor (HGF)-dependent scattering in vitro and tumor growth and metastasis in vivo.
  • 组织特异性Preferentially expressed in metastasizing tumors.
  • 序列相似性Contains 1 SH3 domain.
  • 细胞定位Cytoplasm. Nucleus. Mainly found in the cytoplasm in non-metastasizing tumors.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 7A5 antibody
    • MACC1 antibody
    • MACC1_HUMAN antibody
    • Metastasis associated in colon cancer 1 antibody
    • Metastasis-associated in colon cancer protein 1 antibody
    • OTTHUMP00000201891 antibody
    • Putative binding protein 7a5 antibody
    • SH3 domain-containing protein 7a5 antibody
    • SH3BP4L antibody
    see all

Anti-MACC1 antibody 图像

  • Lane 1 : Anti-MACC1 antibody (ab106579) at 1 µg/ml
    Lane 2 : Anti-MACC1 antibody (ab106579) at 2 µg/ml

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse liver tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 97 kDa
  • Immunohistochemical analysis of MACC1 expression in paraffin embedded Human liver tissue, using ab106579 at 2.5 µg/ml.
  • ICC/IF image of ab106579 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab106579, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunofluorescence of MACC1 in Human Liver cells using ab106579 at 20 ug/ml.

Anti-MACC1 antibody (ab106579)参考文献

This product has been referenced in:
  • Hu H  et al. Metastasis-associated in colon cancer 1 is a novel survival-related biomarker for human patients with renal pelvis carcinoma. PLoS One 9:e100161 (2014). IHC-P ; Human . Read more (PubMed: 24949951) »

See 1 Publication for this product

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