Western blot abreview for Anti-mTOR (phospho S2448) [EPR426(2)] antibody

Good
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Cell lysate - whole cell (placenta)
Specification
placenta
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5%milk in TBS-T
Dilution
1/3000

Secondary antibody

Name
Non-Abcam antibody was used: SC-2030
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000

Detection

Detection method
ECL+
Bands
Specific: 289 kDa Non-specific: 150, 130 kDa
Exposure
5 minute(s) and 0 second(s)
Username

Dr. Varkha Agrawal

Verified customer

提交于 Jul 14 2014

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