Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Testis)
Specification
Testis
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: TBS/BSA/azide
Secondary antibody
Name
Non-Abcam antibody was used: anti Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
I am not familiar with the distribution pattern of this protein.
Results on mouse and rat testis appear very similar.
Positive components in the submitted image are indicated by coloured arrowheads:
red for densely positive nuclei that appear to be confined to the stem cell layer ( immediately above, some spermatogonia exhibit a nuclear speckling)
Yellow indicates a moderate cytoplasmic positivity, more evident in some seminiferous tubules (ST).
Green for a strong apparently cytoplasmic positivity at the lumenal surface of a proportion of ST. To me, this does not appear to be spermatozoa positivity spermatids?Sertoli cells?
Results on mouse and rat testis appear very similar.
Positive components in the submitted image are indicated by coloured arrowheads:
red for densely positive nuclei that appear to be confined to the stem cell layer ( immediately above, some spermatogonia exhibit a nuclear speckling)
Yellow indicates a moderate cytoplasmic positivity, more evident in some seminiferous tubules (ST).
Green for a strong apparently cytoplasmic positivity at the lumenal surface of a proportion of ST. To me, this does not appear to be spermatozoa positivity spermatids?Sertoli cells?
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Carl Hobbs
Verified customer
提交于 Jul 06 2011