Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-mTOR antibody [Y391]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (prostate cancer)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, pH9, 100C 20 min
Permeabilization
No
Specification
prostate cancer
Blocking step
H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde

Other product details

Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent
Dilution
1/1250

Secondary antibody

Name
Non-Abcam antibody was used: Leica Refine anti-mouse anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
automated on Leica Bond III various pre-treatment regimens tested - none, enzyme , heat with citrate, heat with EDTA. EDTA gave best signal to background
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提交于 Apr 24 2015

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