Western blot abreview for Anti-mTOR antibody [Y391] - ChIP Grade

Average
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Keratinocyte)
Loading amount
15 µg
Specification
Keratinocyte
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Other product details

Dilution
1/1000
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS

Secondary antibody

Name
Non-Abcam antibody was used: GE (Amersham) ECL Rabbit IgG, HRP-Linked F(ab')2 F
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
West Pico (Pierce)
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: >175 kDa
Username

Prof. Paul Townsend

Verified customer

提交于 Jun 26 2008

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