Western blot abreview for Anti-mTOR antibody [Y391] - ChIP Grade

Poor
Abreviews
Application
Western blot
Sample
Mouse Tissue lysate - whole (ventricular tissue lysates)
Loading amount
100 µg
Specification
ventricular tissue lysates
Gel Running Conditions
Reduced Denaturing (4 ~ 12% gradient NuPAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Other product details

Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C

Secondary antibody

Name
Non-Abcam antibody was used: Jackson ImmunoResearch anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
SuperSignal WestPico
Exposure
15 hour(s), 0 minute(s) and 0 second(s)
Positive control
30 ug HeLa cell lysates
Negative control
none

Additional data

Additional Notes
The anti-rabbit secondary antibody from Jackson ImmunoResearch we used for this experiment was shown to work well for other rabbit antibodies. The exposure time from 1 min to overnight (15 hours) did not give any band. Whether this is a vial-specific problem remains to be determined.
Abcam response
We are sorry to hear that ab32028 did not work well in your Western Blotting experiment. At this time, we have not received any recent complaints on this product and we are unsure about the cause of the problem. As you indicated, the problem could be vial specific. We would be happy to offer you another vial/lot to test. Please keep us updated with your progress.
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提交于 Jul 05 2008

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