Application
Western blot
Sample
Mouse Cell lysate - nuclear (Embryonic stem cell)
Gel Running Conditions
Non-reduced Denaturing (7%)
Loading amount
60 µg
Specification
Embryonic stem cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking 5% milk, 0.1% Tween-20, TBS
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H + L)-HRP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
30 second(s)
Bands
Specific: 150 kDa
Additional data
Additional Notes
Sin3A was a positive candidate (interactor) in a co-IP experiment.
Lane 1: Input control
Lane 2: IP
Lane 3: IgG control
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 19 2016