Application
Immunoprecipitation
Immuno-precipitation step
Protein A/G
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Total protein in input
40 µg
Other product details
Concentration
4 µg/mg lysate
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: RIPA BUFFER
Western Blot antibody
Name
Non-Abcam antibody was used: anti rabbit secondary antibody produced in goat
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/60000
Additional data
Additional Notes
sin3 plasmid was over expresses in hek 293t cell line and the cells were harvested by using ripa buffer and the whole cell lysate was prepared. one part or the whole cell lysate was subjected to IP. the ip sample is subjected to WB and anti rabbit secondary antibody was used and the band was observed at 150KD
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提交于 Jul 05 2013