Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (8)
Sample
Human Cell lysate - whole cell (HEK293T)
Specification
HEK293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Other product details
Concentration
0.6 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: skimmed milk 5%
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti rabbit antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/60000
Detection
Detection method
ECL
Bands
Specific: 150 kDa
Exposure
5 minute(s) and 0 second(s)
Additional data
Additional Notes
sin3 plasmid was over expresses in hek 293t cell line and the cells were harvested by using ripa buffer and the wholw cell lysate was prepared. one part or the whole cell lysate was subjected to WB. 8%SDS gel was used and 0.6 mirco gram per ML concentration of antibody used and anti rabbit secondary antibody was used and the band was observed at 150KD
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提交于 Jul 04 2013