Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H7.S6 Human ES cells with mCherry gene reporter)
Permeabilization
Yes - 0.1% Triton-X
Specification
H7.S6 Human ES cells with mCherry gene reporter
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
Fixative
Paraformaldehyde
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Alexa Fluor 488 IgG Goat anti Chicken
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 30 2016