Anti-M6PR (cation independent)抗体[MEM-238] (FITC) (ab58841)

概述

  • 产品名称Anti-M6PR (cation independent)抗体[MEM-238] (FITC)
    参阅全部 M6PR (cation independent) 一抗
  • 描述
    小鼠单克隆抗体[MEM-238] to M6PR (cation independent) (FITC)
  • 偶联物FITC. Ex: 493nm, Em: 528nm
  • 经测试应用适用于: ICC/IF, WB, Flow Cytmore details
  • 种属反应性
    与反应: Human
    预测可用于: Non Human Primates
  • 免疫原

    Recombinant Vaccinia virus encoding Mannose 6 Phosphate Receptor (Cation independent) (CD222).

  • 表位ab58841 recognizes an epitope between domains 2 and 5 of Mannose 6 Phosphate Receptor (Cation independent) (CD222).
  • 阳性对照
    • Jurkat whole cell lysate
  • 常规说明

    The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.

性能

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应用

Our Abpromise guarantee covers the use of ab58841 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ICC/IF 1/50.
WB Use at an assay dependent concentration. Predicted molecular weight: 275 kDa. (non-reducing conditions).
Flow Cyt Use 8.3µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

靶标

  • 功能Transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose-6-phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2. Acts as a positive regulator of T-cell coactivation, by binding DPP4.
  • 序列相似性Belongs to the MRL1/IGF2R family.
    Contains 1 fibronectin type-II domain.
  • 结构域Contains 15 repeating units of approximately 147 AA harboring four disulfide bonds each. The most highly conserved region within the repeat consists of a stretch of 13 AA that contains cysteines at both ends.
  • 细胞定位Lysosome membrane. Colocalized with DPP4 in internalized cytoplasmic vesicles adjacent to the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 300 kDa mannose 6 phosphate receptor antibody
    • 300 kDa mannose 6-phosphate receptor antibody
    • Cation independent mannose 6 phosphate receptor antibody
    • Cation-independent mannose-6-phosphate receptor antibody
    • CD222 antibody
    • CD222 antigen antibody
    • CI Man 6 P receptor antibody
    • CI Man-6-P receptor antibody
    • CI MPR antibody
    • CI-M6PR antibody
    • CI-MPR antibody
    • CIMPR antibody
    • IGF 2 receptor antibody
    • IGF 2R antibody
    • IGF II receptor antibody
    • IGF-II receptor antibody
    • IGF2 receptor antibody
    • Igf2r antibody
    • Insulin like growth factor 2 receptor antibody
    • Insulin like growth factor II receptor antibody
    • Insulin-like growth factor 2 receptor antibody
    • Insulin-like growth factor II receptor antibody
    • M6P R antibody
    • M6P/IGF2 receptor antibody
    • M6P/IGF2R antibody
    • M6PR antibody
    • mannose 6 phosphate receptor antibody
    • mannose 6 phosphate receptor, cation independent antibody
    • MPR 300 antibody
    • MPR300 antibody
    • MPRI antibody
    • MPRI_HUMAN antibody
    see all

Anti-M6PR (cation independent) antibody [MEM-238] (FITC) 图像

  • ab58841 staining Mannose 6 Phosphate Receptor in a Human cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% NP-40 + 1% BSA in PBS and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/50 in 0.5% NP-40 + 1% BSA in PBS) for 1 hour at 25°C.

    See Abreview

  • Lane 1 : Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)
    Lane 2 : Mouse IgG1 isotype control

    Lane 1 : Jurkat whole cell lysate
    Lane 2 : Jurkat whole cell lysate


    Predicted band size : 275 kDa
  • Overlay histogram showing HeLa cells stained with ab58841 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58841, 1µg/1x106 cells) for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 (1µg/1x106 cells ). Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde/permeabilized in 0.1% PBS-Tween used under the same conditions.

Anti-M6PR (cation independent) antibody [MEM-238] (FITC) (ab58841)参考文献

This product has been referenced in:
  • Laube F Mannose-6-phosphate/insulin-like growth factor-II receptor in human melanoma cells: effect of ligands and antibodies on the receptor expression. Anticancer Res 29:1383-8 (2009). Inhibition Assay ; Human . Read more (PubMed: 19414391) »
  • Leksa V  et al. The N terminus of mannose 6-phosphate/insulin-like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J Biol Chem 277:40575-82 (2002). WB, Flow Cyt ; Human . Read more (PubMed: 12189157) »

See all 2 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample Human Cell (HEK293, HeLa)
Specification HEK293, HeLa
Permeabilization Yes - 0.5% NP-40, 1%BSA in PBS
Fixative Paraformaldehyde
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Dr. Monica Pons

Verified customer

提交于 Oct 01 2013

Thank you for contacting us. The following two papers include protocols with this clone, MEM-238, which indicate that the antibody recognizes an extracellular epitope. This will also be the case for the FITC conjugate, ab58441. (The unconjugated versio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"