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Recombinant Vaccinia virus encoding Mannose 6 Phosphate Receptor (Cation independent) (CD222).
The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use.
Our Abpromise guarantee covers the use of ab58841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 275 kDa. (non-reducing conditions).|
|Flow Cyt||Use 8.3µl for 106 cells. ab91356-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|
ab58841 staining Mannose 6 Phosphate Receptor in a Human cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% NP-40 + 1% BSA in PBS and blocked with 1% BSA for 10 minutes at 25°C. Samples were incubated with primary antibody (1/50 in 0.5% NP-40 + 1% BSA in PBS) for 1 hour at 25°C.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"