Anti-M13 Bacteriophage Coat蛋白g8p抗体[RL-ph2] (ab9225)
Key features and details
- Mouse monoclonal [RL-ph2] to M13 Bacteriophage Coat Protein g8p
- Suitable for: ICC, Flow Cyt, WB
- Isotype: IgG2a
概述
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产品名称
Anti-M13 Bacteriophage Coat蛋白g8p抗体[RL-ph2] -
描述
小鼠单克隆抗体[RL-ph2] to M13 Bacteriophage Coat蛋白g8p -
宿主
Mouse -
特异性
RL-ph2 reacts with the major M13 filamentous phage coat protein g8p with a molecular weight of 5 kDa.
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经测试应用
适用于: ICC, Flow Cyt, WBmore details -
种属反应性
与反应: Enterobacteria phage M13 -
免疫原
Recombinant full length protein corresponding to Enterobacteria phage M13 M13 Bacteriophage Coat Protein g8p. Isolated M13 phage coat proteins
Database link: P69541 -
常规说明
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
Primary antibody说明
The display of repertoires of antibody fragments on the surface of filamentous phage offers a new way to produce immunoreagents with defined specificities. Phage derived antibody fragments offer a number of advantages over mouse monoclonal antibodies, such as better clearance from the blood, the possibility to select from human combinatorial libraries and the relative ease by which such fragments can be manipulated. The phage display technique thus facilitates the selection of antibody fragments of therapeutic value or research interest. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. -
克隆
单克隆 -
克隆编号
RL-ph2 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
轻链类型
kappa -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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ICC |
Use at an assay dependent concentration.
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Flow Cyt |
1/25 - 1/200.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa).
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说明 |
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ICC
Use at an assay dependent concentration. |
Flow Cyt
1/25 - 1/200. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
1/100 - 1/1000. Detects a band of approximately 5 kDa (predicted molecular weight: 5 kDa). |
靶标
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相关性
M13 is a filamentous bacteriophage composed of circular single stranded DNA (ssDNA) which is 6407 nucleotides long encapsulated in approximately 2700 copies of the major coat protein P8, and capped with 5 copies of two different minor coat proteins (P9, P6, P3) on the ends. The minor coat protein P3 attaches to the receptor at the tip of the F pilus of the host Escherichia coli. Infection with filamentous phages is not lethal, however the infection causes turbid plaques in E. coli. It is a non-lytic virus. However a decrease in the rate of cell growth is seen in the infected cells. Antibodies to M13 filamentous phage coat proteins are instrumental in the selection and detection of phages expressing specific antibody fragments or peptide sequences at their surface. -
别名
- Capsid protein G8P antibody
- Coat protein B antibody
- G8P antibody
see all
图片
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Anti-M13 Bacteriophage Coat Protein g8p antibody [RL-ph2] (ab9225) at 1/1000 dilution + whole cell lysate prepared from XL10Gold cells at 50 µg
Secondary
Rabbit Anti-Mouse IgG H&L (HRP) (ab6728) at 1/2000 dilution
Predicted band size: 5 kDa
Observed band size: 6 kDa why is the actual band size different from the predicted?
Primary antibody incubated for 16 hours at 4°C.Blocking step performed using 5% milk for 1 hour at room temperature.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab9225 被引用在 3 文献中.
- Hulin-Curtis SL et al. Identification of folate receptor a (FRa) binding oligopeptides and their evaluation for targeted virotherapy applications. Cancer Gene Ther 27:785-798 (2020). PubMed: 31902944
- Meulemans EV et al. Phage displayed antibodies specific for a cytoskeletal antigen. Selection by competitive elution with a monoclonal antibody. Hum Antibodies Hybridomas 6:113-8 (1995). PubMed: 8597626
- Meulemans EV et al. Selection of phage-displayed antibodies specific for a cytoskeletal antigen by competitive elution with a monoclonal antibody. J Mol Biol 244:353-60 (1994). PubMed: 7527464