Anti-M13抗体[E1] (Biotin) (ab17269)

概述

  • 产品名称
    Anti-M13抗体[E1] (Biotin)
    参阅全部 M13 一抗
  • 描述
    小鼠单克隆抗体[E1] to M13 (Biotin)
  • 偶联物
    Biotin
  • 特异性
    M13 antibody [E1] (ab17269) is specific for gp3 protein.
  • 经测试应用
    适用于: WB, ELISA, Flow Cytmore details
  • 种属反应性
    M13 antibody [E1] (ab17269) is non-species specific. Not yet tested in other species.
  • 免疫原

    Full length M13 phage coat protein; Exact epitope not determined.

  • 常规说明


    M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯度
    Protein A purified
  • 纯化说明
    M13 antibody [E1] (ab17269) is purified using 0.2µm sterile filter.
  • Primary antibody说明
    M13 antibody [E1] (ab17269) has numerous uses in screening phage display libraries, especially the screening of single chain antibody libraries.
  • 克隆
    单克隆
  • 克隆编号
    E1
  • 骨髓瘤
    unknown
  • 同种型
    IgG2a
  • 轻链类型
    unknown
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab17269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 90 kDa.
ELISA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

靶标

  • 相关性
    The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
  • 别名
    • Bacteriophage M13 antibody
    • Coliphage M13 antibody
    • Enterobacteria phage M13 antibody
    • M13 virus antibody
    • Phage M13 antibody
    see all

文献

ab17269 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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