Anti-M13抗体[E1] (ab24229)

概述

  • 产品名称
    Anti-M13抗体[E1]
    参阅全部 M13 一抗
  • 描述
    小鼠单克隆抗体[E1] to M13
  • 特异性
    Antibody specific for gp3 protein.
  • 经测试应用
    适用于: WB, ELISAmore details
  • 种属反应性
    Reacts with M13 Bacteriophage
  • 免疫原

    M13 bacteriophage.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液
    Preservative: 0.08% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 纯化说明
    Purified over protein A/G column.
  • 克隆
    单克隆
  • 克隆编号
    E1
  • 同种型
    IgG2a
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab24229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明
    ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 45 kDa.
    Sorting of M13 infected cells.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性
      The bacteriophage known as "M13" forms the basis of cloning systems designed to easily introduce mutations into genes inserted into the phage genome. It also has been used in various "phage display" methodologies and combinatorial DNA and peptide libraries. Coat protein A (G3P) is necessary for adsorption of the virion onto the F-pilus of the host cell. There are about five copies of this protein per mature phage. Consists of three domains (N1, N2, and CT). The N2 domain interacts with the F pilus, whereas the N1 domain (connected to N2 by a flexible glycine-rich linker and tightly interacting with it on the phage) forms a complex with the C-terminal domain of tolA at later stages of the infection process. They are located at the adsorption end of the phage particle.
    • 别名
      • Bacteriophage M13 antibody
      • Coliphage M13 antibody
      • Enterobacteria phage M13 antibody
      • M13 virus antibody
      • Phage M13 antibody
      see all

    实验方案

    Anti-M13 antibody [E1] (ab24229)参考文献

    ab24229 has not yet been referenced specifically in any publications.

    Product Wall

    Abreviews
    Application
    ELISA
    Sample
    Bacteria Recombinant protein (filamentous bacteriophage from host E. coli strain)
    Specification
    filamentous bacteriophage from host E. coli strain
    Blocking step
    BSA as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Type
    Indirect
    Username

    Mr. Chang Su Jeon

    Verified customer

    提交于 Aug 13 2015

    Application
    Immunocytochemistry
    Sample
    Bacteria Cultured Cells (Phage Suspension)
    Permeabilization
    No
    Specification
    Phage Suspension
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Fixative
    none
    Username

    Abcam user community

    Verified customer

    提交于 Oct 06 2015

    Yes, the antibody does detect intact phage, at the gp3 protein.

    Thank you for your enquiry. Regarding ab24229 (M13 antibody [E1]), this antibody is specific for p3 but we do not have more epitope information at this time.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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