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ab50370 was raised using fd phages from E. Coli F+ strain (JM109).
Filamentous fd bacteriophages are used in immunoassays for the identification of recombinant antigen- or antibody phages. They have also been used to construct phage-display peptide libraries, which have been useful in selecting peptides that interact with specific domains within target molecules.
Our Abpromise guarantee covers the use of ab50370 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab50370 detecting M13 phage. Samples were blocked with 1% BSA for 2 hours at 25°C and incubated with the primary antibody (1/1000 in 50mM carbonate/bicarbonate buffer, pH 9) for 12 hours at 4°C.