The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50000. Predicted molecular weight: 60 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
1/250 - 1/500.
应用说明Is unsuitable for Flow Cyt.
功能Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
翻译后修饰Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
细胞定位Cell membrane and Secreted > extracellular space.
Yang L et al. Increased expression of colony stimulating factor-1 is a predictor of poor prognosis in patients with clear-cell renal cell carcinoma. BMC Cancer15:67 (2015).
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Wang YW et al. Clinicopathological significance of microRNA-214 in gastric cancer and its effect on cell biological behaviour. PLoS One9:e91307 (2014).
Read more (PubMed: 24614175) »