The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to M-CSF (ab9693). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human MCSF.
Can be used as detection antibody when paired with ab9693.
Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa. The detection limit for recombinant human MCSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate. Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.