Anti-M-CSF抗体(Biotin) (ab84262)

概述

性能

应用

Our Abpromise guarantee covers the use of ab84262 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 0.25 - 1 µg/ml. Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human MCSF.
Sandwich ELISA Use a concentration of 0.25 - 1 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to M-CSF (ab9693). Allows the detection of at least 0.2 - 0.4 ng/well of recombinant human MCSF. Can be used as detection antibody when paired with ab9693.
WB Use a concentration of 0.1 - 0.2 µg/ml. Predicted molecular weight: 60 kDa. The detection limit for recombinant human MCSF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

靶标

  • 功能
    Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.
  • 翻译后修饰
    Glycosylation and proteolytic cleavage yield different soluble forms. A high molecular weight soluble form is a proteoglycan containing chondroitin sulfate.
    Isoform 1 is N- and O-glycosylated. Isoform 3 is N-glycosylated.
  • 细胞定位
    Cell membrane and Secreted > extracellular space.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Colony stimulating factor 1 (macrophage) antibody
    • Colony stimulating factor 1 antibody
    • Colony stimulating factor macrophage specific antibody
    • CSF 1 antibody
    • CSF-1 antibody
    • CSF1 antibody
    • CSF1_HUMAN antibody
    • Csfm antibody
    • Lanimostim antibody
    • M CSF antibody
    • M-CSF antibody
    • Macrophage Colony Stimulating Factor 1 antibody
    • Macrophage colony stimulating factor antibody
    • MCSF antibody
    • MGC31930 antibody
    • Processed macrophage colony-stimulating factor 1 antibody
    see all

实验方案

文献

ab84262 has not yet been referenced specifically in any publications.

客户评价及客户问答

Application
Flow Cytometry
Sample
Mouse Cell (Two cell lines: L929 and CMG12-14)
Permeabilization
Yes - BD fix/perm and perm/wash buffers for intracellular cytokine staining
Gating Strategy
live cells, singlets
Specification
Two cell lines: L929 and CMG12-14
Preparation
Cell harvesting/tissue preparation method: cells were grown in tissue culture and harvested by trypsinization
Sample buffer: BD fixation and permeabilization buffer set
Fixation
Paraformaldehyde
Username

Mary Fontana

Verified customer

提交于 Oct 30 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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