Anti-LZTR1抗体(ab106655)

概述

  • 产品名称
  • 描述
    兔多克隆抗体to LZTR1
  • 特异性
    At least four isoforms of LZTR1 are known to exist; ab106655 will recognize all but the shortest isoform. ab106655 is predicted not to cross-react with LZTR2.
  • 经测试应用
    适用于: IHC-P, WB, ICC/IFmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A 14 amino acid synthetic peptide near the N terminus of Human LZTR1 (CAJ86451).

  • 阳性对照
    • Human heart tissue lysate; Mouse heart tissue This antibody gave a positive result when used in the following formaldehyde fixed cell lines: NIH-3T3.

性能

应用

Our Abpromise guarantee covers the use of ab106655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 95 kDa.
ICC/IF Use a concentration of 1 µg/ml.

靶标

图片

  • ICC/IF image of ab106655 stained NIH-3T3 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab106655 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Lane 1 : Anti-LZTR1 antibody (ab106655) at 1 µg/ml
    Lane 2 : Anti-LZTR1 antibody (ab106655) at 2 µg/ml

    Lane 1 : Human heart tissue lysate
    Lane 2 : Human heart tissue lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 95 kDa
  • ab106655, at 5 µg/ml, staining LZTR1 in Mouse heart tissue by Immunohistochemistry.

文献

ab106655 has not yet been referenced specifically in any publications.

客户评价及客户问答

The sequences we are referring to are accession numbers CAJ86451 840 aa Theoretical pI/Mw: 6.12 / 94718.90 BAH12325 821 aa Theoretical pI/Mw: 6.06 / 92393.15 BAC86816 799aa Theoretical pI/Mw: 6.20 / 90586.39 BAA07508 552aa Theoret...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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