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Our Abpromise guarantee covers the use of ab14917 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 1 - 15 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.|
|IHC-Fr||Use a concentration of 1 - 5 µg/ml.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 22590615|
|Flow Cyt||Use at an assay dependent concentration. PubMed: 22590615
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC - Wholemount||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.
This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.
ab14917 at 1/100 staining mouse tissue sections (E17.5 lymphatic vessels by inferior vena cava) by IHC-Fr. The tissue was acetone fixed and incubated with the antibody for 2 hours in 1% BSA in PBS. An Alexa Fluor ® 488 conjugated donkey anti-rabbit IgG antibody was used as the secondary.
ab14917 staining LYVE1 in Rabbit spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in Fa.Zytomed) for 24 hours at 4°C. An undiluted Biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.
ab14917 staining LYVE1 in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-Buffer pH9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.
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