概述

  • 产品名称Anti-LYVE1抗体
    参阅全部 LYVE1 一抗
  • 描述
    兔多克隆抗体to LYVE1
  • 经测试应用适用于: ELISA, WB, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Human
  • 免疫原

    Recombinant fragment corresponding to LYVE1.

性能

应用

Our Abpromise guarantee covers the use of ab14917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22590615
Flow Cyt Use at an assay dependent concentration. PubMed: 22590615

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC - Wholemount Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • 组织特异性Mainly expressed in endothelial cells lining lymphatic vessels.
  • 序列相似性Contains 1 Link domain.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Anti-LYVE1 antibody 图像

  • ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.

    See Abreview

  •  ab14917 at 1/100 staining mouse tissue sections (E17.5 lymphatic vessels by inferior vena cava) by IHC-Fr. The tissue was acetone fixed and incubated with the antibody for 2 hours in 1% BSA in PBS. An Alexa Fluor ® 488 conjugated donkey anti-rabbit IgG antibody was used as the secondary.

    See Abreview

  • ab14917 staining LYVE1 in Rabbit spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in Fa.Zytomed) for 24 hours at 4°C. An undiluted Biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab14917 staining LYVE1 in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-Buffer pH9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.

    See Abreview

Anti-LYVE1 antibody (ab14917)参考文献

This product has been referenced in:
  • Gurung HR  et al. Cornea lymphatics drive the CD8(+) T-cell response to herpes simplex virus-1. Immunol Cell Biol 95:87-98 (2017). ICC/IF ; Mouse . Read more (PubMed: 27577867) »
  • Zhu G  et al. Lipopolysaccharide increases the release of VEGF-C that enhances cell motility and promotes lymphangiogenesis and lymphatic metastasis through the TLR4- NF-?B/JNK pathways in colorectal cancer. Oncotarget 7:73711-73724 (2016). IHC ; Human . Read more (PubMed: 27713159) »

See all 103 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Infmammed lung tissue)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization No
Specification Infmammed lung tissue
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Fixative Paraformaldehyde
Username

Dr. Jim Hsiao

Verified customer

提交于 Jun 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application IHC - Wholemount
Sample Mouse Embryo (dermal lymphatic vessels)
Specification dermal lymphatic vessels
Username

Abcam user community

Verified customer

提交于 Dec 14 2016

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Lungs)
Permeabilization Yes - 0.5% TX100
Specification Lungs
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Oct 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse embryo, E14.5)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Specification Mouse embryo, E14.5
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Molly Plehaty

Verified customer

提交于 Sep 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 27 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Syrian Hamster Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (jejunum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification jejunum
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Uterus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification Uterus
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 21 2015

Application Western blot
Sample Mouse Tissue lysate - whole (Heart and brain)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 10 µg
Treatment Tissue was mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification Heart and brain
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

提交于 Apr 27 2015

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