概述

  • 产品名称Anti-LYVE1抗体
    参阅全部 LYVE1 一抗
  • 描述
    兔多克隆抗体to LYVE1
  • 经测试应用适用于: ELISA, WB, IHC-Fr, ICC/IF, Flow Cyt, IHC - Wholemount, IHC-Pmore details
  • 种属反应性
    与反应: Mouse, Rabbit, Human
  • 免疫原

    Recombinant fragment corresponding to LYVE1.

性能

应用

Our Abpromise guarantee covers the use of ab14917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
ELISA Use a concentration of 1 - 15 µg/ml.
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 35 kDa.
IHC-Fr Use a concentration of 1 - 5 µg/ml.
ICC/IF Use at an assay dependent concentration. PubMed: 22590615
Flow Cyt Use at an assay dependent concentration. PubMed: 22590615

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC - Wholemount Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.

靶标

  • 功能Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • 组织特异性Mainly expressed in endothelial cells lining lymphatic vessels.
  • 序列相似性Contains 1 Link domain.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Anti-LYVE1 antibody 图像

  • ab14917 at a 1/100 dilution staining LYVE1 from mouse tuberculosis infected lung by immunohistochemistry (paraffin-embedded sections). The antibody was incubated with the tissue for 30 minutes and then detected with an HRP conjugated goat anti-rabbit antibody.

    This image is courtesy of an Abreview submitted by Elizabeth Chlipala on 18 November 2005.

    See Abreview

  •  ab14917 at 1/100 staining mouse tissue sections (E17.5 lymphatic vessels by inferior vena cava) by IHC-Fr. The tissue was acetone fixed and incubated with the antibody for 2 hours in 1% BSA in PBS. An Alexa Fluor ® 488 conjugated donkey anti-rabbit IgG antibody was used as the secondary.

    See Abreview

  • ab14917 staining LYVE1 in Rabbit spleen tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% BSA for 30 minutes at 20°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/200 in Fa.Zytomed) for 24 hours at 4°C. An undiluted Biotin-conjugated anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • ab14917 staining LYVE1 in Mouse uterus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% serum for 30 minutes at 20°C; antigen retrieval was by heat mediation in a EDTA-Buffer pH9.0. Samples were incubated with primary antibody (1/50 in PBS) for 12 hours at 20°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab14917 at 1/2000 dilution staining Ha-Ras transgenic mouse bladder (cancer) by Immunohistochemistry (Formalin-fixed paraffin-embedded sections). The tissue was formaldehyde fixed and blocked with serum prior to incubation with the primary antibody for 12 hours. A biotinylated polyclonal antibody was used as the secondary.

    See Abreview

Anti-LYVE1 antibody (ab14917)参考文���

This product has been referenced in:
  • Nemska S  et al. Revascularization of the graft in obliterative bronchiolitis after heterotopic tracheal transplantation. Physiol Rep 4:N/A (2016). IHC-P ; Mouse . Read more (PubMed: 26908711) »
  • Kwon JW  et al. Comparison of postoperative corneal changes between dry eye and non-dry eye in a murine cataract surgery model. Int J Ophthalmol 9:218-24 (2016). IHC ; Mouse . Read more (PubMed: 26949638) »

See all 96 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Lungs)
Permeabilization Yes - 0.5% TX100
Specification Lungs
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Paraformaldehyde
Username

Herr Dr. Vladimir Milenkovic

Verified customer

提交于 Oct 10 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Mouse embryo, E14.5)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0
Permeabilization No
Specification Mouse embryo, E14.5
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative Paraformaldehyde
Username

Molly Plehaty

Verified customer

提交于 Sep 15 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Skin)
Antigen retrieval step Enzymatic
Permeabilization No
Specification Skin
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 May 12 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (lung)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification lung
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 27 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Syrian Hamster Tissue sections (skin)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification skin
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (jejunum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification jejunum
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Jan 22 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (Uterus)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA-Puffer pH 9,0
Permeabilization No
Specification Uterus
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

提交于 Sep 21 2015

Application Western blot
Sample Mouse Tissue lysate - whole (Heart and brain)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 10 µg
Treatment Tissue was mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification Heart and brain
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

提交于 Apr 27 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate Buffer
Sample Pig Tissue sections (Lung Biopsy)
Specification Lung Biopsy
Permeabilization No
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

提交于 Mar 11 2015

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer ph=6
Sample Mouse Tissue sections (Lung)
Specification Lung
Permeabilization No
Fixative Formaldehyde
Username

Dr. Francois Daubeuf

Verified customer

提交于 Mar 06 2015

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"