概述

  • 产品名称Anti-LYVE1抗体
    参阅全部 LYVE1 一抗
  • 描述
    兔多克隆抗体to LYVE1
  • 经测试应用适用于: IHC-Fr, Flow Cyt, IHC-P, WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Rat
  • 免疫原

    Recombinant fragment corresponding to Human LYVE1 aa 24-232. Recombinant human soluble LYVE1 fragment produced in insect cells (ab54341).
    Database link: Q9Y5Y7
    (Peptide available as ab54341)

  • 阳性对照
    • human colon carcinoma

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.40
    Constituent: PBS
  • Concentration information loading...
  • 纯度Protein A purified
  • 纯化说明Protein-A Chromatography (+his tag depleted).
  • Primary antibody说明The lymphatic vasculature forms a second circulatory system that drains extracellular fluid from the tissues and provides an exclusive environment in which immune cells can encounter and respond to foreign antigen. Recently a number of interesting molecules have been identified that may be exploited as markers for lymphatic endothelium, including the hyaluronan receptor LYVE1, PALE, VEGFR3, podoplanin.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab10278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 6 - 30 µg/ml.

Fix sections for 10 min at -20°C in MeOH.

Flow Cyt Use at an assay dependent concentration.
IHC-P Use a concentration of 2 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 35-45 kDa (predicted molecular weight: 35 kDa).Can be blocked with Recombinant Human LYVE1 protein (ab54341).

靶标

  • 功能Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as a hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
  • 组织特异性Mainly expressed in endothelial cells lining lymphatic vessels.
  • 序列相似性Contains 1 Link domain.
  • 翻译后修饰O-glycosylated.
  • 细胞定位Membrane. Localized to the plasma membrane and in vesicles near extranuclear membranes which may represent trans-Golgi network (TGN) and endosomes/prelysosomeal compartments. Undergoes ligand-dependent internalization and recycling at the cell surface.
  • Information by UniProt
  • 数据库链接
  • 别名
    • Cell surface retention sequence-binding protein 1 antibody
    • CRSBP 1 antibody
    • CRSBP-1 antibody
    • CRSBP1 antibody
    • extracellular link domain containing 1 antibody
    • extracellular link domain-containing 1 antibody
    • Extracellular link domain-containing protein 1 antibody
    • HAR antibody
    • Hyaluronic acid receptor antibody
    • Lymphatic endothelium specific hyaluronan receptor antibody
    • lymphatic vessel endothelial hyaluronan receptor 1 antibody
    • Lymphatic vessel endothelial hyaluronic acid receptor 1 antibody
    • LYVE 1 antibody
    • LYVE-1 antibody
    • LYVE1 antibody
    • LYVE1_HUMAN antibody
    • XLKD1 antibody
    see all

Anti-LYVE1 antibody 图像

  • Immunohistochemistry (Frozen sections) analysis of human colon carcinoma tissue sections labelling LYVE1 with ab10278.

  • ab10278 (2µg/ml) staining LYVE1 in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is lymphatic endothelium staining of lymphatic ducts where blood vessel endothelium and smooth muscle is wholly negative.
    Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • All lanes : Anti-LYVE1 antibody (ab10278) at 1 µg/ml

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 22 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 4 minutes
    LYVE-1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • Flow Cytometry analysis of human dermal microvascular endothelial cells (HDMVEC) labelling LYVE1 with ab10278.

  • All lanes : Anti-LYVE1 antibody (ab10278)

    Lane 1 : Recombinant Human LYVE1 protein (ab54341)
    Lane 2 : Recombinant Mouse LYVE1 protein (ab54342)


    Predicted band size : 35 kDa
  • Rat skin was fixed with paraformaldehyde in 15% saturated picric acid solution for 4hr. Prior to sectioning, the specimen was infiltrated in O.C.T. and frozen in isopentane. The frozen specimen was sectioned these were rinsed in PBS for 15 min to remove O.C.T. and incubated in a 3% sodium deoxycholate solution. The specimens were rinsed twice with distilled water and then with PBS three times. The sections were incubated in 10% normal goat serum for 12 hr at 4°C, then for 12 hr with ab10278. After washing with PBS, the specimens were incubated with Alexa Fluor® 555-conjugated goat anti-rabbit IgG (H+L) (1:500),  for 12 hr at 4°C. The cell nuclei were counterstained with YoYo-1. Images were obtained by using confocal microscope.

    See Abreview

Anti-LYVE1 antibody (ab10278)参考文献

This product has been referenced in:
  • Klar AS  et al. Analysis of blood and lymph vascularization patterns in tissue-engineered human dermo-epidermal skin analogs of different pigmentation. Pediatr Surg Int 30:223-31 (2014). Human . Read more (PubMed: 24363089) »
  • Tonthat CV & Di Girolamo N Nestin expression in pterygia: potential role in angiogenesis. Br J Ophthalmol N/A:N/A (2013). Read more (PubMed: 24008823) »

See all 4 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cynomolgus Monkey Tissue sections (ileum)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Tris-based buffer, pH8
Permeabilization No
Specification ileum
Blocking step Hydrogen Peroxide in PBS as blocking agent for 12 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

提交于 Nov 04 2016

Thank you for your interest in our LYVE1 antibody ab10278. We do not have any small samples but our guarantee applies to IHC on paraffin-embedded or frozen human tissue. We do not have any data for prostate tissue stained with ab10278 but LYVE1 has bee...

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Thank you for your inquiry.

We did not have an MSDS beforehands, as the product does not contain any chemicals requiring an MSDS. However we have uploaded now the MSDS for you - you can find it now on the website. http://www.abcam.com/index.h...

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Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (skin)
Specification skin
Fixative Paraformaldehyde
Blocking step Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 10
Username

Dr. Vyacheslav Ogay

Verified customer

提交于 May 14 2007

My apologies for this problem. Some people indeed have had problems with this antibody in IHC-P (this application came from a customer review) and we believe the antibody may be difficult to optimise, hence we have removed this application from the dat...

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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (various)
Specification various
Fixative Formaldehyde
Antigen retrieval step Heat mediated
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 2.5
Username

Abcam user community

Verified customer

提交于 Jul 05 2006

Thank you for your enquiry. I am sorry to hear that you have been experiencing difficulties with this antibody. I have read through your technical questionaire and I have a few comments. The application of immunohistochemistry was added to the li...

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Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rabbit has...

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Thank you for your patience and I'm sorry to hear that you are experiencing difficulty with ab10278. Originally, it has not been tested on formalin-fixed tissue sections. However, recently one of our customers has used it successfully on this type of a...

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Thank you for your enquiry. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Should you...

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