Anti-Lysyl tRNA synthetase抗体(ab91595)

概述

  • 产品名称Anti-Lysyl tRNA synthetase抗体
    参阅全部 Lysyl tRNA synthetase 一抗
  • 描述
    兔多克隆抗体to Lysyl tRNA synthetase
  • 经测试应用适用于: IHC-Pmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a region between residue 550 and the C-terminus (residue 597) of Human Lysyl tRNA synthetase (NP_005539.1).

  • 阳性对照
    • Human Breast Carcinoma, Colon Carcinoma

性能

应用

Our Abpromise guarantee covers the use of ab91595 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
  • 应用说明IHC-P: 1/100 - 1/500. Epitope exposure is recommended. Epitope exposure with Tris-EDTA pH9.0 buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 功能Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
    • 疾病相关Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
    • 序列相似性Belongs to the class-II aminoacyl-tRNA synthetase family.
    • 结构域The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
    • 细胞定位Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
    • Information by UniProt
    • 数据库链接
    • 别名
      • CMTRIB antibody
      • DFNB89 antibody
      • EC 6.1.1.6 antibody
      • KARS 1 antibody
      • KARS 2 antibody
      • KARS antibody
      • KARS1 antibody
      • KARS2 antibody
      • KIAA0070 antibody
      • KRS antibody
      • Lysine tRNA ligase antibody
      • Lysine--tRNA ligase antibody
      • LysRS antibody
      • Lysyl-tRNA synthetase antibody
      • SYK_HUMAN antibody
      see all

    Anti-Lysyl tRNA synthetase antibody 图像

    • ab91595, at 1/100 dilution, staining Lysyl tRNA synthetase in formalin-fixed paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry using Red-fluorescent goat anti-rabbit IgG highly cross-adsorbed Antibody Hilyte PlusTM 555 secondary antibody at a dilution of 1/100
    • ab91595, at 1/250 dilution, staining Lysyl tRNA synthetase in formalin-fixed paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry using DAB staining.
    • ab91595, at 1/250 dilution, staining Lysyl tRNA synthetase in formalin-fixed paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry using DAB staining.

    Anti-Lysyl tRNA synthetase antibody (ab91595)参考文献

    ab91595 has not yet been referenced specifically in any publications.

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