Anti-Lysyl tRNA synthetase抗体(ab70524)


  • 产品名称Anti-Lysyl tRNA synthetase抗体
    参阅全部 Lysyl tRNA synthetase 一抗
  • 描述
    兔多克隆抗体to Lysyl tRNA synthetase
  • 特异性We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • 经测试应用适用于: IHC-P, ICC, WB, IPmore details
  • 种属反应性
    与反应: Human
    预测可用于: Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • 免疫原

    A region between residue 550 and the C-terminus (residue 597) of human Lysyl tRNA synthetase (NP_005539.1).

  • 阳性对照
    • Whole cell lysate from HeLa and 293T cells.




Our Abpromise guarantee covers the use of ab70524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.

For formalin-fixed paraffin-embedded cells, epitope retrieval with Tris-EDTA pH9.0 is recommended.

WB 1/2000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 66 kDa).
IP Use at 1-4 µg/mg of lysate.


  • 功能Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation.
  • 疾病相关Defects in KARS are the cause of Charcot-Marie-Tooth disease recessive intermediate type B (CMTRIB) [MIM:613641]; also called Charcot-Marie-Tooth neuropathy recessive intermediate B. CMTRIB is a form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Recessive intermediate forms of Charcot-Marie-Tooth disease are characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec.
  • 序列相似性Belongs to the class-II aminoacyl-tRNA synthetase family.
  • 结构域The N-terminal domain (1-65) of the cytoplasmic isoform is a functional tRNA-binding domain (By similarity), is required for nuclear localization, is involved in the interaction with DARS, but has a repulsive role in the binding to EEF1A1. A central domain (208-259) is involved in homodimerization and is required for interaction with HIV-1 GAG and incorporation into virions. The C-terminal domain (452-597) is not required for interaction with AIMP2.
  • 细胞定位Mitochondrion and Cytoplasm. Nucleus. Cell membrane. Secreted. Secretion is induced by TNF-alpha.
  • Information by UniProt
  • 数据库链接
  • 别名
    • CMTRIB antibody
    • DFNB89 antibody
    • EC antibody
    • KARS 1 antibody
    • KARS 2 antibody
    • KARS antibody
    • KARS1 antibody
    • KARS2 antibody
    • KIAA0070 antibody
    • KRS antibody
    • Lysine tRNA ligase antibody
    • Lysine--tRNA ligase antibody
    • LysRS antibody
    • Lysyl-tRNA synthetase antibody
    • SYK_HUMAN antibody
    see all

Anti-Lysyl tRNA synthetase antibody 图像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling Lysyl tRNA synthetase with ab70524 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Lysyl tRNA synthetase antibody (ab70524) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    developed using the ECL technique

    Predicted band size : 66 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human Lysyl tRNA synthetase by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab70524 at 3 µg/mg for IP (Lane 1) and at 1 µg/ml for subsequent WB detection. Lane 2 represents control IgG IP.

Anti-Lysyl tRNA synthetase antibody (ab70524)参考文献

ab70524 has not yet been referenced specifically in any publications.

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