概述
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产品名称Anti-Lysozyme抗体
参阅全部 Lysozyme 一抗 -
描述兔多克隆抗体to Lysozyme
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宿主Rabbit
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经测试应用适用于: IHC-P, IHC-Fr, ICCmore details
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种属反应性与反应: Human
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免疫原
Purified human Lysozyme from the urine of patients with monocytic leukemia.
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阳性对照
- Human tonsil tissue. IF/ICC: A549 cell line.
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常规说明
ab76784 labels myeloid cells, histocytes, granulocytes, macrophages and monocytes. It is helpful in detection of myeloid or monocytic nature of acute leukemia.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.05% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
纯度Protein A purified
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Primary antibody说明ab76784 labels myeloid cells, histocytes, granulocytes, macrophages and monocytes. It is helpful in detection of myeloid or monocytic nature of acute leukemia.
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克隆多克隆
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同种型IgG
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研究领域
相关产品
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab76784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/50 - 1/200. | |
| IHC-Fr | 1/50 - 1/200. | |
| ICC | 1/50 - 1/200. |
靶标
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功能Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
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疾病相关Amyloidosis 8
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序列相似性Belongs to the glycosyl hydrolase 22 family.
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细胞定位Secreted.
- Information by UniProt
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数据库链接
- Entrez Gene: 4069 Human
- Omim: 153450 Human
- SwissProt: P61626 Human
- Unigene: 524579 Human
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别名
- 1 4 beta N acetylmuramidase C antibody
- 1 antibody
- 4-beta-N-acetylmuramidase C antibody
see all
图片
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Immunocytochemistry/ Immunofluorescence - Anti-Lysozyme antibody (ab76784)ICC/IF image of ab76784 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76784, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
文献
ab76784 has not yet been referenced specifically in any publications.
客户评价及客户问答
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"