概述

  • 产品名称Anti-Lysozyme抗体
    参阅全部 Lysozyme 一抗
  • 描述
    兔多克隆抗体to Lysozyme
  • 经测试应用适用于: IHC-P, IHC-Fr, ICCmore details
  • 种属反应性
    与反应: Human
  • 免疫原

    Purified human Lysozyme from the urine of patients with monocytic leukemia.

  • 阳性对照
    • Human tonsil tissue. IF/ICC: A549 cell line.
  • 常规说明


    ab76784 labels myeloid cells, histocytes, granulocytes, macrophages and monocytes. It is helpful in detection of myeloid or monocytic nature of acute leukemia.

性能

应用

Our Abpromise guarantee covers the use of ab76784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/200.
IHC-Fr 1/50 - 1/200.
ICC 1/50 - 1/200.

靶标

  • 功能Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • 疾病相关Amyloidosis 8
  • 序列相似性Belongs to the glycosyl hydrolase 22 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 4 beta N acetylmuramidase C antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • LYZ antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Anti-Lysozyme antibody 图像

  • ICC/IF image of ab76784 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76784, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-Lysozyme antibody (ab76784)参考文献

ab76784 has not yet been referenced specifically in any publications.

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