概述

  • 产品名称Anti-Lysozyme抗体
    参阅全部 Lysozyme 一抗
  • 描述
    兔多克隆抗体to Lysozyme
  • 特异性Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Lysozyme from hen egg white.
  • 经测试应用适用于: WB, IP, ELISA, ICCmore details
  • 种属反应性
    与反应: Chicken
  • 免疫原

    Lysozyme from Hen Egg White

  • 阳性对照
    • Assayed against 1.0 ug of Lysozyme from hen egg white in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.878% Sodium chloride, 0.424% Potassium phosphate
  • Concentration information loading...
  • 纯度IgG fraction
  • 纯化说明IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Lysozyme.
  • 克隆多克隆
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/500 - 1/2000.
IP 1/100.
ELISA 1/25000.
ICC 1/500 - 1/2000.

靶标

  • 功能Lysozymes have primarily a bacteriolytic function; those in tissues and body fluids are associated with the monocyte-macrophage system and enhance the activity of immunoagents.
  • 疾病相关Amyloidosis 8
  • 序列相似性Belongs to the glycosyl hydrolase 22 family.
  • 细胞定位Secreted.
  • Information by UniProt
  • 数据库链接
  • 别名
    • 1 4 beta N acetylmuramidase C antibody
    • 1 antibody
    • 4-beta-N-acetylmuramidase C antibody
    • EC 3.2.1.17 antibody
    • LYSC_HUMAN antibody
    • Lysosyme antibody
    • Lysozyme (renal amyloidosis) antibody
    • Lysozyme C antibody
    • Lysozyme C precursor antibody
    • LYZ antibody
    • LZM antibody
    • Renal amyloidosis antibody
    see all

Anti-Lysozyme antibody 图像

  • Anti-Lysozyme antibody (ab391) at 1/1000 dilution + Lysozyme (Hen Egg White) at 0.05 µg

    Secondary
    Rabbit HRP conjugate at 1/40000 dilution

Anti-Lysozyme antibody (ab391)参考文献

This product has been referenced in:
  • Doulias PT  et al. Mass spectrometry-based identification of S-nitrosocysteine in vivo using organic mercury assisted enrichment. Methods : (2012). WB . Read more (PubMed: 23116708) »
  • Sagiv Y  et al. A distal effect of microsomal triglyceride transfer protein deficiency on the lysosomal recycling of CD1d. J Exp Med 204:921-8 (2007). WB . Read more (PubMed: 17403933) »

See all 2 Publications for this product

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Sample Chicken Tissue sections (embryo)
Specification embryo
Permeabilization No
Fixative Paraformaldehyde
Username

Anna Keyle

Verified customer

提交于 Jun 12 2014

Thank you for your enquiry. We currently do not have a lysozyme antibody conjugated to AP and we are unable to custom conjugate antibodies.

This antibody has not yet been tested for use with IHC. Should you decide to go ahead and test this antibody, please let us know how you get on, and in return we will forward a USD15/EUR15/GBP10 Amazon gift voucher. If you have more questions, please c...

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Yes, this antibody has been tested by immunoprecipitation and Western blot. The suggested working dilution is 1/4000 to 1/10000, although optimisation in your own lab is recommended.

Polyclonal antibodies are normally produced in SPF (specific pathogen free) rabbits and these rabbits are free of infection with E. coli. Even with these precautions, quite a lot of polyclonal antibodies give cross-reaction with E. coli proteins. Try ...

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This antibody is known to work in immunoblotting, however it's reactivity against dog lysozyme has not been determined. As the antibody was raised against lysozyme from chicken egg white, it is uncertain whether it will react with canine.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"