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Lysozyme isolated from urine of patients with monocytic leukemia.
Our Abpromise guarantee covers the use of ab2408 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 15 kDa (predicted molecular weight: 17 kDa).|
|IHC-P||1/50 - 1/100.|
|IHC-Fr||Use at an assay dependent concentration.|
Lysozyme was immunoprecipitated using 0.5mg HL60 whole cell extract, 5µg of Rabbit polyclonal to Lysozyme and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, HL60 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab2408.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 16kDa; Lysozyme
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