Anti-Lysosomal acid lipase/LAL抗体[9G7F12] (ab36597)
Key features and details
- Mouse monoclonal [9G7F12] to Lysosomal acid lipase/LAL
- Suitable for: Flow Cyt, WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2a
概述
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产品名称
Anti-Lysosomal acid lipase/LAL抗体[9G7F12]
参阅全部 Lysosomal acid lipase/LAL 一抗 -
描述
小鼠单克隆抗体[9G7F12] to Lysosomal acid lipase/LAL -
宿主
Mouse -
经测试应用
适用于: Flow Cyt, WB, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant fragment corresponding to Lysosomal acid lipase/LAL.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from tissue culture supernatant. -
克隆
单克隆 -
克隆编号
9G7F12 -
同种型
IgG2a -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab36597于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use 0.5µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB | (1) |
1/500 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa).
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IHC-P |
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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说明 |
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Flow Cyt
Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
1/500 - 1/2000. Detects a band of approximately 45 kDa (predicted molecular weight: 46 kDa). |
IHC-P
Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
靶标
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功能
Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. -
疾病相关
Defects in LIPA are the cause of Wolman disease (WOD) [MIM:278000]. WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year.
Defects in LIPA are the cause of cholesteryl ester storage disease (CESD) [MIM:278000]. CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. -
序列相似性
Belongs to the AB hydrolase superfamily. Lipase family. -
细胞定位
Lysosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3988 Human
- Omim: 278000 Human
- SwissProt: P38571 Human
- Unigene: 643030 Human
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别名
- Acid cholesteryl ester hydrolase antibody
- CESD antibody
- cholesterol ester hydrolase antibody
see all
图片
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All lanes : Anti-Lysosomal acid lipase/LAL antibody [9G7F12] (ab36597)
Lane 1 : Molecular weight marker
Lane 2 : Truncated LAL recombinant protein
Predicted band size: 46 kDa
Observed band size: ~45 kDa why is the actual band size different from the predicted? -
ab36597 (2 µg/ml) staining Lysosomal acid lipase/LAL in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is lysosomal staining in the bronchiolar epithelium and resident macrophages .
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required. -
Overlay histogram showing HepG2 cells stained with ab36597 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab36597, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab36597 被引用在 3 文献中.
- Cui W et al. Lipophagy-derived fatty acids undergo extracellular efflux via lysosomal exocytosis. Autophagy 17:690-705 (2021). PubMed: 32070194
- Cahova M et al. The increased activity of liver lysosomal lipase in nonalcoholic Fatty liver disease contributes to the development of hepatic insulin resistance. Biochem Res Int 2012:135723 (2012). PubMed: 21904679
- Wang F et al. Role of lysosomal acid lipase in the intracellular metabolism of LDL-transported dehydroepiandrosterone-fatty acyl esters. Am J Physiol Endocrinol Metab 295:E1455-61 (2008). WB ; Human . PubMed: 18796546