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RabMAb

Anti-Lysophospholipase 1抗体[EPR3666] (ab91600)

概述

  • 产品名称
    Anti-Lysophospholipase 1抗体[EPR3666]
    参阅全部 Lysophospholipase 1 一抗
  • 描述
    兔单克隆抗体[EPR3666] to Lysophospholipase 1
  • 经测试应用
    适用于: WB, ICC, Flow Cytmore details
    不适用于: IHC-P or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues in Human Lysophospholipase 1.

  • 阳性对照
    • Human fetal liver, Human fetal heart and Human spleen cell lysates.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液
    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 50% Glycerol, 0.05% BSA
  • 纯度
    Tissue culture supernatant
  • 克隆
    单克隆
  • 克隆编号
    EPR3666
  • 同种型
    IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab91600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/2000. Detects a band of approximately 21, 24 kDa (predicted molecular weight: 25 kDa).
ICC 1/500 - 1/1000.
Flow Cyt 1/50.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • 应用说明
    Is unsuitable for IHC-P or IP.
  • 靶标

    Anti-Lysophospholipase 1 antibody [EPR3666] 图像



    • Predicted band size : 25 kDa

      Lane 1: Wild type HAP1 whole cell lysate (40 µg)
      Lane 2: LYPLA1 knockout HAP1 whole cell lysate (40 µg)
      Lane 3: Mouse spleen whole cell lysate (40 µg)
      Lanes 1 - 3: Merged signal (red and green). Green - ab91600 observed at 25 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab91600 was shown to specifically react with LYPLA1 when LYPLA1 knockout samples were used. Wild-type and LYPLA1 knockout samples were subjected to SDS-PAGE. ab91600 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10000 dilution respectively. Blots were developed with ab216773, Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed and ab216776, Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • All lanes : Anti-Lysophospholipase 1 antibody [EPR3666] (ab91600) at 1/1000 dilution

      Lane 1 : Human fetal liver lysate
      Lane 2 : Human fetal heart lysate
      Lane 3 : Human spleen lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-rabbit HRP at 1/2000 dilution

      Predicted band size : 25 kDa

    Anti-Lysophospholipase 1 antibody [EPR3666] (ab91600)参考文献

    This product has been referenced in:
    • Vujic I  et al. Acyl protein thioesterase 1 and 2 (APT-1, APT-2) inhibitors palmostatin B, ML348 and ML349 have different effects on NRAS mutant melanoma cells. Oncotarget 7:7297-306 (2016). WB . Read more (PubMed: 26771141) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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