概述

  • 产品名称Anti-Lys 20p + 21p抗体[31F5]
  • 描述
    小鼠单克隆抗体[31F5] to Lys 20p + 21p
  • 经测试应用适用于: WB, ICC/IFmore details
  • 种属反应性
    与反应: Saccharomyces cerevisiae
  • 免疫原

    This was raised against a yeast nucleolar prep, and screened by immunocytochemistry and western blotting. Nucleolar prep contains Lys20p protein (as well as Nop1p/fibrillarin).

  • 常规说明


    Lys20p and Lys21p are isozymes of yeast homocitrate synthase. They are the products of two distinct genes producing proteins of molecular weight 47.1 kDa in the case of Lys20p and 48.6kDa for Lys21p. However Lys20p and Lys21p are ~90% identical at the amino acid level and MCA-31F5 detects both proteins, revealing a doublet of stained bands on blots.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液Preservative: 10mM Sodium Azide
    Constituents: Raw Ascites
  • Concentration information loading...
  • 纯度Ascites
  • 纯化说明Sterile filtered.
  • Primary antibody说明Lys20p and Lys21p are isozymes of yeast homocitrate synthase. They are the products of two distinct genes producing proteins of molecular weight 47.1 kDa in the case of Lys20p and 48.6kDa for Lys21p. However Lys20p and Lys21p are ~90% identical at the amino acid level and MCA-31F5 detects both proteins, revealing a doublet of stained bands on blots.
  • 克隆单克隆
  • 克隆编号31F5
  • 同种型IgG
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 47.1, 48.6 kDa.

For yeast protein samples, ECL detection, 1/10000 - 1/50000. Detects bands of approximately 47.1 kDa Lys 20p and 48.6 kDa Lys 21p. For non-ECL detection, 1/1000 - 1/5000.

ICC/IF 1/1000 - 1/10000.

Yeast cells.

Anti-Lys 20p + 21p antibody [31F5] (ab4574)参考文献

This product has been referenced in:
  • DeLuna A  et al. Need-based up-regulation of protein levels in response to deletion of their duplicate genes. PLoS Biol 8:e1000347 (2010). WB ; Saccharomyces cerevisiae . Read more (PubMed: 20361019) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"