The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
应用说明Is unsuitable for Flow Cyt or IP.
功能Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
组织特异性Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
细胞定位Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor®488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).
ab124789 at 1/100 dilution staining LYRIC in paraffin-embedded Human brain tissues by Immunohistochemistry.
Western blot - Anti-LYRIC antibody [EP4445] (ab124789)
All lanes : Anti-LYRIC antibody [EP4445] (ab124789) at 1/10000 dilution
Lane 1 : HeLa cell lysate Lane 2 : Jurkat cell lysate Lane 3 : PC3 cell lysate Lane 4 : PC12 cell lysate Lane 5 : NIH 3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size : 64 kDa
Anti-LYRIC antibody [EP4445] (ab124789)参考文献
This product has been referenced in:
Li M et al. Astrocyte elevated gene-1 promotes the proliferation and invasion of breast cancer cells by activating the Wnt/ß-catenin signaling pathway. Oncol Lett13:2385-2390 (2017).
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