概述

  • 产品名称
    Anti-LYRIC抗体[EP4445]
    参阅全部 LYRIC 一抗
  • 描述
    兔单克隆抗体[EP4445] to LYRIC
  • 经测试应用
    适用于: WB, IHC-P, ICC/IFmore details
    不适用于: Flow Cyt or IP
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to residues in Human LYRIC.

  • 阳性对照
    • HeLa, Jurkat, PC3, PC12 and NIH 3T3 cell lysates; Human brain and breast tissues; HeLa cells.
  • 常规说明

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124789 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 64 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min.
ICC/IF 1/500.
  • 应用说明
    Is unsuitable for Flow Cyt or IP.
  • 靶标

    • 功能
      Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.
    • 组织特异性
      Widely expressed with highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Overexpressed in various cancers including breast, brain, prostate, melanoma and glioblastoma multiforme.
    • 细胞定位
      Endoplasmic reticulum membrane. Nucleus membrane. Cell junction > tight junction. Nucleus > nucleolus. Cytoplasm > perinuclear region. In epithelial cells, recruited to tight junctions (TJ) during the maturation of the TJ complexes. A nucleolar staining may be due to nuclear targeting of an isoform lacking the transmembrane domain (By similarity). TNF-alpha causes translocation from the cytoplasm to the nucleus.
    • Information by UniProt
    • 数据库链接
    • 别名
      • 3D3 antibody
      • 3D3/LYRIC antibody
      • AEG 1 antibody
      • AEG-1 antibody
      • AEG1 antibody
      • Astrocyte elevated gene 1 antibody
      • Astrocyte elevated gene-1 protein antibody
      • LYRIC antibody
      • LYRIC/3D3 antibody
      • LYRIC_HUMAN antibody
      • Lysine rich CEACAM1 associated protein antibody
      • Lysine rich CEACAM1 co isolated protein antibody
      • Lysine-rich CEACAM1 co-isolated protein antibody
      • Metadherin antibody
      • Metastasis adhesion protein antibody
      • MTDH antibody
      • Protein LYRIC antibody
      see all

    图片

    • ab124789 at 1/100 dilution staining LYRIC in paraffin-embedded Human breast tissues by Immunohistochemistry.
    • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling LYRIC with purified ab124789 at a dilution of 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei counterstained with DAPI (blue).

      Control: PBS only.

    • ab124789 at 1/100 dilution staining LYRIC in paraffin-embedded Human brain tissues by Immunohistochemistry.
    • All lanes : Anti-LYRIC antibody [EP4445] (ab124789) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : PC3 cell lysate
      Lane 4 : PC12 cell lysate
      Lane 5 : NIH 3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size : 64 kDa

    文献

    This product has been referenced in:
    • Li M  et al. Astrocyte elevated gene-1 promotes the proliferation and invasion of breast cancer cells by activating the Wnt/ß-catenin signaling pathway. Oncol Lett 13:2385-2390 (2017). WB ; Human . Read more (PubMed: 28454408) »
    • Du Y  et al. Metadherin regulates actin cytoskeletal remodeling and enhances human gastric cancer metastasis via epithelial-mesenchymal transition. Int J Oncol 51:63-74 (2017). WB, IHC-P ; Human . Read more (PubMed: 28534938) »

    See all 3 Publications for this product

    客户评价及客户问答

    There are currently no Customer reviews or Questions for ab124789.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    注册