产品名称 Anti-LYPLAL1抗体 参阅全部 LYPLAL1 一抗
WB, ELISA, IHC-Fr more details
Rat, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Orangutan
DRFKITNDCPEHLE , corresponding to internal sequence amino acids 79-92 of Human LYPLAL1 (NP_620149.1)
Human Adipose and Heart lysates.
存放说明 Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles.
存储溶液 Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
Concentration information loading...
纯度 Immunogen affinity purified
纯化说明 ab77640 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用说明 Peptide ELISA: Use at an assay dependent dilution. Antibody detection limit dilution: 1/16000.
WB: Use at a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 26 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
序列相似性 Belongs to the AB hydrolase 2 family.
Information by UniProt
Anti-LYPLAL1 antibody 图像
Western blot - LYPLAL1 antibody (ab77640)
Immunohistochemistry (Frozen sections) - Anti-LYPLAL1 antibody (ab77640) Image from Fan Q et al., PLoS Genet. 2012 Jun;8(6):e1002753. Epub 2012 Jun 7.Fig 5.; doi:10.1371/journal.pgen.1002753; June 7, 2012, PLoS Genet 8(6): e1002753.
Immunofluorescence analysis of mouse retina, retinal pigment epithelium and sclera in induced myopic eye (bottom left), fellow non-occluded eye (bottom right), and independent control eye (top right). LYPLAL1 (green) was stained using ab77640 at 1/200 dilution. A fluorescein-conjugated rabbit anti-goat secondary antibody was used at 1/800 dilution. Nuclei were stained with DAPI (blue).
Negative control eye (top left) was treated with the secondary antibody alone and DAPI.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"