重组Anti-Lyn (phospho Y507)抗体[EP504Y] (ab33914)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP504Y] to Lyn (phospho Y507)
- Suitable for: Dot blot, WB, IP
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Lyn (phospho Y507)抗体[EP504Y]
参阅全部 Lyn 一抗 -
描述
兔单克隆抗体[EP504Y] to Lyn (phospho Y507) -
宿主
Rabbit -
经测试应用
适用于: Dot blot, WB, IPmore details
不适用于: Flow Cyt or ICC/IF -
种属反应性
与反应: Human
预测可用于: Mouse, Rat -
免疫原
Synthetic peptide within Human Lyn (phospho Y507). The exact sequence is proprietary.
Database link: P07948 -
阳性对照
- WB: HeLa (untreated and treated with pervanadate) and Raji treated with pervanadate whole cell lysates. IP: SH-SY5Y treated with pervandate whole cell lysate.
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EP504Y -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab33914于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Dot blot |
1/1000.
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WB |
1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa).
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IP |
1/80.
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说明 |
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Dot blot
1/1000. |
WB
1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa). |
IP
1/80. |
靶标
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功能
Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents. -
组织特异性
Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors. -
序列相似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
结构域
The protein kinase domain plays an important role in its localization in the cell membrane. -
翻译后修饰
Ubiquitinated. Ubiquitination is SH3-dependent. -
细胞定位
Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport. - Information by UniProt
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数据库链接
- Entrez Gene: 4067 Human
- Entrez Gene: 17096 Mouse
- Entrez Gene: 81515 Rat
- Omim: 165120 Human
- SwissProt: P07948 Human
- SwissProt: P25911 Mouse
- SwissProt: Q07014 Rat
- Unigene: 491767 Human
see all -
形式
This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request. -
别名
- AA407514 antibody
- EC 2.7.10.2 antibody
- FLJ26625 antibody
see all
图片
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All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/1000 dilution
Lane 1 : Raji whole cell lysate
Lane 2 : Raji treated with 1mM pervanadate for 30 min whole cell lysate
Lane 3 : Raji treated with 1mM pervanadate for 30 min whole cell lysate, the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 61 kDa
Observed band size: 61 kDaBlocking and dilution buffer: 5% NFDM/TBST.
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Purified ab33914 at 1/80 dilution (2µg) immunoprecipitating Lyn in SH-SY5Y treated with pervandate whole cell lysate.
Lane 1 (input): SH-SY5Y (Human neuroblastoma epithelial cell) treated with pervandate whole cell lysate 10µg
Lane 2 (+): ab33914 + SH-SY5Y treated with pervandate whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab256224 in SH-SY5Y treated with pervandate whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 61 kDa -
Dot blot analysis of Lyn (phospho Y507) phospho peptide (Lane 1) and Lyn non-phospho peptide (Lane 2) labelling Lyn (phospho Y507) with ab33914 at a dilution of 1/1000. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/100,000.
Blocking and dilution buffer: 5% NFDM /TBST.
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All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/5000 dilution
Lane 1 : 10ug Non treated HeLa cell lysate
Lane 2 : 10ug HeLa cell lysate treated with Pervanadate
Secondary
All lanes : Goat anti-rabbit HRP labeled.
Predicted band size: 61 kDa
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab33914 被引用在 11 文献中.
- Sheng L et al. Overexpression of Fc?RIIB regulates downstream protein phosphorylation and suppresses B cell activation to ameliorate systemic lupus erythematosus. Int J Mol Med 46:1409-1422 (2020). PubMed: 32945349
- Xu Y et al. MicroRNA-218-5p inhibits cell growth and metastasis in cervical cancer via LYN/NF-?B signaling pathway. Cancer Cell Int 18:198 (2018). PubMed: 30524205
- Gavali S et al. LYN, a key mediator in estrogen-dependent suppression of osteoclast differentiation, survival, and function. Biochim Biophys Acta Mol Basis Dis 1865:547-557 (2018). PubMed: 30579930
- Tondera C et al. Insights into binding of S100 proteins to scavenger receptors: class B scavenger receptor CD36 binds S100A12 with high affinity. Amino Acids 49:183-191 (2017). PubMed: 27734162
- Sharma N et al. SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis. J Immunol 192:4859-66 (2014). PubMed: 24733849