RabMAb

Anti-Lyn (phospho Y507)抗体[EP504Y] (ab33914)

概述

  • 产品名称Anti-Lyn (phospho Y507)抗体[EP504Y]
    参阅全部 Lyn 一抗
  • 描述
    兔单克隆抗体[EP504Y] to Lyn (phospho Y507)
  • 特异性The antibody will detect Lyn’s phosphorylation on Tyrosine 507.
  • 经测试应用适用于: IHC-P, WB, IPmore details
    不适用于: Flow Cyt or ICC/IF
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Tyrosine 507 of Lyn (Human).

  • 阳性对照
    • HeLa cell lysate (un-treated and treated with Pervanadate)
  • 常规说明

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

  • 形式Liquid
  • 存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • 存储溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 纯度Tissue culture supernatant
  • 克隆单克隆
  • 克隆编号EP504Y
  • 同种型IgG
  • 研究领域

相关产品

应用

Our Abpromise guarantee covers the use of ab33914 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/50 - 1/100.
WB 1/500 - 1/5000. Detects a band of approximately 53-56 kDa (predicted molecular weight: 61 kDa).
IP 1/50.
  • 应用说明Is unsuitable for Flow Cyt or ICC/IF.
  • 靶标

    • 功能Down regulates expression of stem cell growth factor receptor (KIT). Acts as an effector of EpoR (erythropoietin receptor) in controlling KIT expression and may play a central role in erythroid differentiation during the switch between proliferation and maturation (By similarity). Acts as a positive regulator of cell movement while negatively regulating adhesion to stromal cells by inhibiting the ICAM-1-binding activity of beta-2 integrins. Acts as the mediator that relays suppressing signals from the chemokine receptor CXCR4 to beta-2 integrin LFA-1 in hematopoietic precursors. Involved in induction of stress-activated protein kinase (SAPK), but not ERK or p38 MAPK, in response to genotoxic agents. Induces SAPK by a MKK7- and MEKK1-dependent mechanism. The LYN -> MEKK1 -> MKK7 -> SAPK pathway is functional in the induction of apoptosis by genotoxic agents.
    • 组织特异性Widely expressed in a variety of organs, tissues, and cell types such as epidermoid, hematopoietic, and neuronal cells. Expressed in primary neuroblastoma tumors.
    • 序列相似性Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH2 domain.
      Contains 1 SH3 domain.
    • 结构域The protein kinase domain plays an important role in its localization in the cell membrane.
    • 翻译后修饰Ubiquitinated. Ubiquitination is SH3-dependent.
    • 细胞定位Cell membrane. Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Golgi apparatus. Accumulates in the nucleus by inhibition of CRM1-mediated nuclear export. Nuclear accumulation is increased by inhibition of its kinase activity. The trafficking from the Golgi apparatus to the plasma membrane occurs in a kinase domain-dependent but kinase activity independent manner and is mediated by exocytic vesicular transport.
    • Information by UniProt
    • 数据库链接
    • 形式This protein is known to be similar in amino acid sequence to HCK (P08631), LCK (P06239), FYN (P06241), YES1 (P07947), and SRC (P12931). Therefore, cross-reactivity with these homologous proteins may be observed. We would be happy to provide immunogen alignment information upon request.
    • 别名
      • AA407514 antibody
      • EC 2.7.10.2 antibody
      • FLJ26625 antibody
      • Hck 2 antibody
      • JTK 8 antibody
      • JTK8 antibody
      • Lck/Yes related novel protein tyrosine kinase antibody
      • LYN antibody
      • LYN proto oncogene, Src family tyrosine kinase antibody
      • LYN_HUMAN antibody
      • ONCOGENE LYN antibody
      • p53Lyn antibody
      • p56Lyn antibody
      • Tyrosine protein kinase LYN antibody
      • Tyrosine-protein kinase Lyn antibody
      • V yes 1 Yamaguchi sarcoma viral related oncogene homolog antibody
      • Yamaguchi sarcoma viral (v yes 1) related oncogene homolog antibody
      see all

    Anti-Lyn (phospho Y507) antibody [EP504Y] 图像

    • All lanes : Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914) at 1/5000 dilution

      Lane 1 : 10ug Non treated HeLa cell lysate
      Lane 2 : 10ug HeLa cell lysate treated with Pervanadate

      Secondary
      Goat anti-rabbit HRP labeled.

      Predicted band size : 61 kDa

    Anti-Lyn (phospho Y507) antibody [EP504Y] (ab33914)参考文献

    This product has been referenced in:
    • Sharma N  et al. SHP2 phosphatase promotes mast cell chemotaxis toward stem cell factor via enhancing activation of the Lyn/Vav/Rac signaling axis. J Immunol 192:4859-66 (2014). Read more (PubMed: 24733849) »
    • Luo W  et al. A balance between B cell receptor and inhibitory receptor signaling controls plasma cell differentiation by maintaining optimal Ets1 levels. J Immunol 193:909-20 (2014). Read more (PubMed: 24929000) »

    See all 7 Publications for this product

    Product Wall

    Thank you for your enquiry.

    These 2 antibodies have lot specific concentrations. If you could please forward me the lot numbers for ab40660 and ab33914 I'd be happy to enquire about the concentrations.

    Thanks for the additional info...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"