概述

  • 产品名称Anti-Lyl1抗体
    参阅全部 Lyl1 一抗
  • 描述
    兔多克隆抗体to Lyl1
  • 经测试应用适用于: WBmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse Lyl1.

    (Peptide available as ab30337.)

  • 阳性对照
    • ab31726 gave a positive result in WB with MOLT4 and THP1 whole cell lysates.

性能

应用

Our Abpromise guarantee covers the use of ab31726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).

靶标

Anti-Lyl1 antibody 图像

  • All lanes : Anti-Lyl1 antibody (ab31726) at 1 µg/ml

    Lane 1 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 28 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)


    Exposure time : 4 minutes

Anti-Lyl1 antibody (ab31726)参考文献

ab31726 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (OCI/AML-2)
Loading amount 60 µg
Specification OCI/AML-2
Gel Running Conditions Reduced Denaturing (12)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

提交于 Jun 01 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application ChIP
Sample Mouse Cell lysate - nuclear (haematopoietic progenitor cell line HPC-7)
Specification haematopoietic progenitor cell line HPC-7
Type Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step Real-time PCR
Positive control i) a competitor antibody
ii) 2 regulatory regions known to bind this transcription factor
Negative control a regulatory region known to be devoid of this transcription factor
Username

Abcam user community

Verified customer

提交于 Oct 05 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"