Synthetic peptide conjugated to KLH derived from within residues 250 to the C-terminus of Mouse Lyl1.
(Peptide available as ab30337.)
- ab31726 gave a positive result in WB with MOLT4 and THP1 whole cell lysates.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa).
Note=A chromosomal aberration involving LYL1 may be a cause of a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(7;19)(q35;p13) with TCRB.
Contains 1 bHLH (basic helix-loop-helix) domain.
Information by UniProt
- bHLHa18 antibody
- Class A basic helix-loop-helix protein 18 antibody
- Lyl1 antibody
Western blot - Anti-Lyl1 antibody (ab31726)
All lanes :
Anti-Lyl1 antibody (ab31726) at 1 µg/mlLane 1 :
MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell LysateLane 2 :
THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.SecondaryAll lanes :
Goat Anti-Rabbit IgG H&L (HRP) (ab97051
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.Predicted band size:
28 kDaObserved band size:
30 kDa (why is the actual band size different from the predicted?
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"