概述

  • 产品名称Anti-LYK5抗体
    参阅全部 LYK5 一抗
  • 描述
    小鼠单克隆抗体to LYK5
  • 经测试应用适用于: WB, ELISAmore details
  • 种属反应性
    与反应: Human
    预测可用于: Mouse, Rat, Cow, Cynomolgus Monkey, Orangutan
  • 免疫原

    Recombinant fragment: LLEKLNGTVP CLLDTSTIPA EELTMSPSRS VANSGLSDSL TTSTPRPSNG DSPSHPYHRT FSPHFHHFVE QCLQRNPDAR YPCWPGPGLR ESRGCS, corresponding to amino acids 251-346 of human LYK5 with a 26 kDa tag (NP_699166).

  • 阳性对照
    • HeLa nuclear cell lysate; Recombinant tagged fragment of human LYK5 (the immunogen).

性能

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应用

Our Abpromise guarantee covers the use of ab77016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB
ELISA
  • 应用说明ELISA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight for isoform 2: 39 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Members of the STE-20 like kinase family are known to stimulate MAPK pathways by directly activating MAPKKK. LYK5 is a novel pseudokinase member of this family consisting of a STE-20 like kinase domain but lacks several residues that are required for its catalytic activity. It specifically binds LKB1 and plays a key role in regulating the tumor suppressor activities of LKB1. It functions as an upstream activator of LKB1 and also directs the sub-cellular localization of LKB1 by anchoring it in the cytoplasm. LYK5-LKB1 interaction results in phosphorylation of LYK5 and enhanced autophosphorylation of LKB1.
    • 细胞定位Cytoplasmic and Nuclear
    • 数据库链接
    • 别名
      • Protein kinase LYK5 antibody
      • Serologically defined breast cancer antigen NY BR 96 antibody
      • STE20 related adapter protein antibody
      • STRAD alpha antibody
      • STRAD antibody
      see all

    Anti-LYK5 antibody 图像

    • Anti-LYK5 antibody (ab77016) at 5 µg/ml + recombinant fragment of human LYK5 at 0.2 µg

      Secondary
      Goat anti-Mouse IgG (H&L)-HRP at 1/5000 dilution

      Predicted band size : 39 kDa
      Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    • Anti-LYK5 antibody (ab77016) at 1/500 dilution + HeLa cell lysate at 25 µg

      Secondary
      Goat anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 39 kDa
      Observed band size : 36 kDa (why is the actual band size different from the predicted?)

    Anti-LYK5 antibody (ab77016)参考文献

    ab77016 has not yet been referenced specifically in any publications.

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