Anti-Ly76抗体[TER-119] (Phycoerythrin) (ab93586)

概述

  • 产品名称Anti-Ly76抗体[TER-119] (Phycoerythrin)
    参阅全部 Ly76 一抗
  • 描述
    大鼠单克隆抗体[TER-119] to Ly76 (Phycoerythrin)
  • 偶联物Phycoerythrin. Ex: 488nm, Em: 575nm
  • 经测试应用适用于: Flow Cytmore details
  • 种属反应性
    与反应: Mouse
  • 免疫原

    Tissue/ cell preparation - Mouse fetal liver cells

  • 阳性对照
    • BALB/c bone marrow cells

性能

应用

Our Abpromise guarantee covers the use of ab93586 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt
  • 应用说明Flow Cyt: Use at a concentration of 0.25 µg/test.
    A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • 靶标

    • 相关性Ly76 is a glycophorin A-associated protein. It is an erythroid-specific antigen expressed on early proerythroblasts to mature erythrocytes, but not BFU-E or CFU-E.
    • 细胞定位Plasma membrane
    • 数据库链接
    • 别名
      • Ly 76 antibody
      • Lymphocyte antigen 76 antibody
      • TER 119 antibody
      • TER119 antibody

    Anti-Ly76 antibody [TER-119] (Phycoerythrin) 图像

    • flow cytometric analysis showing staining of BALB/c bone marrow cells with 0.125 µg of PE Rat IgG2b Isotype Control (open histogram) or 0.125 µg of ab93586 (colored histogram).

    Anti-Ly76 antibody [TER-119] (Phycoerythrin) (ab93586)参考文献

    This product has been referenced in:
    • Kina T  et al. The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage. Br J Haematol 109:280-7 (2000). Read more (PubMed: 10848813) »
    • Vannucchi AM  et al. Identification and characterization of a bipotent (erythroid and megakaryocytic) cell precursor from the spleen of phenylhydrazine-treated mice. Blood 95:2559-68 (2000). Read more (PubMed: 10753835) »

    See all 2 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Flow Cytometry
    Sample Mouse Cell (Erythrocytes)
    Specification Erythrocytes
    Preparation Cell harvesting/tissue preparation method: Isolation of purified erythrocytes from whole blood using percoll gradient
    Sample buffer: PBS with 5mM EDTA
    Fixation Unfixed
    Permeabilization No
    Gating Strategy live cell population of purified erythrocytes gated on the basis of FSC/SSC. P2 gating for positive cell population was set so that <1% of the negative cell population was PE positive.
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    提交于 Mar 25 2011

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"