ab93583 at 0.25 µg staining Ly76 in C57Bl/6 bone marrow cells by Flow Cytometry.
Left; FITC anti-mouse CD45 and 0.25 µg of PE-Cy7 Rat IgG2b Iso Cntrl.
Right; 0.25 µg of ab93583.
Total viable cells were used for analysis.
A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.