RabMAb

Anti-LY75抗体[EPR5233] (ab124897)

概述

  • 产品名称Anti-LY75抗体[EPR5233]
    参阅全部 LY75 一抗
  • 描述
    兔单克隆抗体[EPR5233] to LY75
  • 经测试应用适用于: WB, IP, IHC-P, ICCmore details
  • 种属反应性
    与反应: Mouse, Human
    不与反应: Rat
  • 免疫原

    Synthetic peptide corresponding to residues in the intracellular domain of Human LY75.

  • 阳性对照
    • Human tonsil tissue; Human thymus, lymph node, tonsil and Mouse thymus lysates.
  • 常规说明

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

性能

应用

Our Abpromise guarantee covers the use of ab124897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/1000 - 1/10000. Detects a band of approximately 205 kDa (predicted molecular weight: 198 kDa).
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/100 - 1/250.

靶标

  • 功能Acts as an endocytic receptor to direct captured antigens from the extracellular space to a specialized antigen-processing compartment (By similarity). Causes reduced proliferation of B-lymphocytes.
  • 组织特异性Expressed in spleen, thymus, colon and peripheral blood lymphocytes. Detected in myeloid and B lymphoid cell lines. Isoform 2 and isoform 3 are expressed in malignant Hodgkin lymphoma cells called Hodgkin and Reed-Sternberg (HRS) cells.
  • 序列相似性Contains 10 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • 翻译后修饰N-glycosylated.
  • 细胞定位Membrane.
  • Information by UniProt
  • 数据库链接
  • 别名
    • C-type lectin domain family 13 member B antibody
    • CD 205 antibody
    • CD205 antibody
    • CD205 antigen antibody
    • CLEC 13B antibody
    • CLEC13B antibody
    • DEC 205 antibody
    • DEC-205 antibody
    • DEC205 antibody
    • gp200 antibody
    • gp200 MR6 antibody
    • gp200-MR6 antibody
    • LY 75 antibody
    • Ly-75 antibody
    • LY75 antibody
    • LY75_HUMAN antibody
    • Lymphocyte antigen 75 antibody
    • Renal Cell Carcinoma (gp200) antibody
    see all

Anti-LY75 antibody [EPR5233] 图像

  • ab124897, at 1/250 dilution, staining LY75 in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-LY75 antibody [EPR5233] (ab124897) at 1/1000 dilution

    Lane 1 : Human thymus lysate
    Lane 2 : Human lymph node lysate
    Lane 3 : Human tonsil lysate
    Lane 4 : Mouse thymus lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size : 198 kDa
  • ab124897 showing negative staining in Normal kidney tissue.

  • ab124897 showing negative staining in Normal brain tissue.

  • ab124897 showing positive staining in Normal thymus tissue.

  • ab124897 showing positive staining in Normal spleen tissue.

  • ab124897 showing negative staining in Skeletal muscle tissue.

  • ab124897 showing positive staining in Normal colon lymphocytes tissue.

  • ab124897 showing negative staining in Normal lung tissue.

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

Anti-LY75 antibody [EPR5233] (ab124897)参考文献

This product has been referenced in:
  • D'Auria KM  et al. In vivo physiological and transcriptional profiling reveals host responses to Clostridium difficile toxin A and toxin B. Infect Immun 81:3814-24 (2013). Read more (PubMed: 23897615) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (thymus cortex)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: sodium citrate buffer pH6
Permeabilization Yes - Tween 20 (0,1% in TBS)
Specification thymus cortex
Blocking step BSA as blocking agent for 40 minute(s) · Concentration: 1% · Temperature: 20°C
Fixative Formaldehyde
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提交于 Mar 02 2016

Thank you for your reply.

I am pleased to hear you would like to participate in the testing discount scheme. As requested, I have generated the discount code for your as follows:

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Thank you for contacting us yesterday in regards to the anti-LY75 antibody (ab124897).

I now have some further information to share with you. The antibody has been tested in ICC ofRamos cells and the protocol used is attached to this email. I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"